Rabbit anti-Mouse IFN-gamma Monoclonal Antibody for Flow Cytometry (Intracellular) - P01580

Core Product Specifications and Parameters

Biological Background: IFN-gamma Function and Localization

• Type II interferon primarily produced by activated T-cells and NK cells, with pivotal roles in antimicrobial, antiviral, and antitumor immune responses. Related references: PMID:11585387 PMID:8456301
• Signals through the JAK-STAT pathway upon binding to its receptor IFNGR1, leading to STAT1 activation and transcription of IFN-gamma-regulated genes.
• Enhances antigen presentation by inducing a shift from constitutive proteasome subunits to immunoproteasome subunits, thereby increasing the quantity and quality of MHC class I peptides.
• Up-regulates MHC class II complexes on the cell surface by promoting expression of cathepsins B, H, and L.
• Participates in the regulation of hematopoietic stem cell development, quiescence, and differentiation. Related references: PMID:20535209 PMID:25078851
• Secreted glycoprotein that acts as a cytokine, classified under antiviral defense, growth regulation, and signal transduction.
• Released primarily from activated T lymphocytes, consistent with its role in adaptive immunity.
• UniProt molecular weight: 17,907 Da; observed product weight: 18 kDa (consistent with glycosylation).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length mature mouse IFN-gamma (amino acids 1-155), suggesting recognition of native secreted IFN-gamma; consider validating detection of intracellular cytokine in relevant cell types.
• For intracellular flow cytometry, optimise fixation and permeabilization conditions for the specific cell sample; the conjugate may require protocol adjustments to balance signal intensity and cell integrity.
• As a rabbit monoclonal antibody, it typically offers high specificity; include appropriate isotype controls and, if multiplexing, check for fluorophore compatibility.
• Verify performance in your own experimental system, as antibody performance can vary with cell stimulation conditions and staining protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials and technical consultation, covering every stage from concept to clinic. As a one-stop procurement partner, we provide validated antibody pairs, optimised monoclonal antibodies, bulk ancillary reagents, and rare target sourcing for immunological targets such as IFN-gamma. Our supply capabilities are tailored to streamline your development of in‑vitro diagnostic tests and research assays.