Rabbit anti-Mouse IFN-gamma Monoclonal Antibody for FC (intra) - P01580

Core Product Specifications and Parameters

Biological Background: Interferon gamma Function and Localization

• Interferon gamma (IFNG) is a type II interferon cytokine primarily produced by activated T lymphocytes and NK cells.
• It plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Related references: PMID:11585387, PMID:8456301
• IFNG signals through the JAK-STAT pathway via IFNGR1, leading to STAT1 activation and transcription of IFNG-regulated genes, including IRF1.
• It enhances MHC class I antigen presentation by inducing immunoproteasome subunits and activator PA28, and up-regulates MHC class II complexes via cathepsins B, H, and L (By similarity).
• Participates in hematopoietic stem cell regulation, affecting development, quiescence, and differentiation. Related references: PMID:20535209, PMID:25078851
• Secreted glycoprotein with a molecular weight of approximately 18 kDa, widely used as a marker for Th1 responses.

Experimental Guidance and Technical Tips

• The immunogen (full‐length recombinant mouse IFNG) supports the antibody’s suitability for intracellular staining; consider optimizing fixation and permeabilization protocols for your cell type.
• Since IFNG is secreted, stimulation with PMA/ionomycin in the presence of a protein transport inhibitor (e.g., brefeldin A) is typically required to accumulate cytokine for detection by flow cytometry.
• Validate antibody specificity and optimal titer using IFNG‐deficient mouse cells or recombinant IFNG blocking experiments in your relevant sample system.
• For multi‐color panels, ensure compensation is properly set, especially when using other PE‐ or APC‐conjugated antibodies due to the brightness of .

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with validated IVD raw materials, including this IFN‑gamma monoclonal antibody for immuno‑oncology and infectious disease research. We offer bulk recombinant antibody supplies, optimized antibody pairs, and ancillary reagents for seamless assay development. Contact us to source rare‑target raw materials and accelerate your diagnostic pipeline with confidence.