Rabbit anti-Mouse ICOS/CD278 Monoclonal Antibody for Flow Cytometry - Q9WVS0

Core Product Specifications and Parameters

Biological Background: ICOS/CD278 Function and Localization

• ICOS (Inducible T-cell costimulator, CD278), also designated AILIM, CRP-1, and CCLP, is a stimulatory receptor expressed on activated and antigen-experienced T-cells (PubMed:11343123). Related references: PMID:11343123
• Upon binding ICOSL on antigen-presenting cells, ICOS delivers costimulatory signals that enhance T-cell proliferation, lymphokine secretion (e.g., IL-10), and help for antibody secretion by B-cells (PubMed:10657606). Related references: PMID:10657606
• Mechanistically, ICOS potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling, and can also activate PI3K signaling independently of calcium flux (PubMed:27693916, PubMed:19915142). Related references: PMID:27693916 PMID:19915142
• ICOS is essential for efficient T-cell–B-cell interaction, normal antibody responses to T-cell-dependent antigens, and CD40-mediated immunoglobulin class switching (PubMed:11343122). Related references: PMID:11343122
• ICOS plays a critical role in the differentiation of T follicular regulatory cells during immune challenges such as viral infection (PubMed:36754569). Related references: PMID:36754569
• Subcellularly, ICOS is a single-pass type I membrane protein localized to the cell membrane; it is highly expressed in thymic medulla, lymph node germinal centers, and Peyer patches, with low levels in spleen.
• Structurally, ICOS contains an extracellular immunoglobulin domain, a disulfide bond, and an N-glycosylation site, and possesses a transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen (aa 21-142) covers the extracellular domain, enabling recognition of native surface ICOS on live cells.
• This -conjugated monoclonal antibody is ready for direct flow cytometry; titrate to determine optimal concentration and include appropriate controls (e.g., isotype, compensation beads).
• ICOS is upregulated upon T-cell activation; consider stimulating cells (e.g., with anti-CD3/CD28) to enhance detection.
• For intracellular staining, permeabilization protocols may compromise surface signal; validate in the relevant mouse sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of IVD raw materials, offering diagnostic developers and researchers a consolidated source for high-quality immunoreagents. This anti-mouse ICOS monoclonal antibody exemplifies our catalog of immunology-focused tools, including optimized monoclonal/polyclonal antibodies, validated antibody pairs, and bulk ancillary reagents. For rare-target sourcing or custom requirements, our team bridges the gap from concept to clinic—contact us to discuss your workflow needs.