Rabbit anti-Mouse ICOS/CD278 Monoclonal Antibody for Flow Cytometry - Q9WVS0

Core Product Specifications and Parameters

Biological Background: ICOS/CD278 Function and Localization

• ICOS (Inducible T-cell costimulator), also known as AILIM, CCLP, CRP-1, and Ly115, is a stimulatory receptor expressed on activated or antigen-experienced T-cells. It is encoded by the Icos gene (GeneID 54167 in mouse) and belongs to the immunoglobulin superfamily.
• Upon binding its ligand ICOSL on antigen-presenting cells, ICOS delivers costimulatory signals that enhance T-cell proliferation, lymphokine secretion (e.g., IL-10), up-regulation of cell-cell interaction molecules, and B-cell antibody responses [PMID:10657606]. Related references: PMID:10657606
• ICOS plays a critical role in T follicular regulatory cell differentiation during immune challenges such as viral infection [PMID:36754569]. Related references: PMID:36754569
• Mechanistically, ICOS potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling, and independently activates PI3K signaling [PMID:27693916, PMID:19915142]. Related references: PMID:27693916 PMID:19915142
• ICOS is essential for efficient T-B cell interaction and normal antibody responses to T-cell dependent antigens, preventing apoptosis of pre-activated T-cells and facilitating CD40-mediated immunoglobulin class switching [PMID:11343122]. Related references: PMID:11343122
• Subcellularly, ICOS is a transmembrane protein localized at the cell membrane, featuring a signal peptide, an immunoglobulin domain, a transmembrane helix, and disulfide bonds; it is glycosylated.
• ICOS is highly expressed in the thymic medulla, germinal centers and T-cell zones of lymph nodes and Peyer's patches, and at low levels in the spleen, consistent with its role in activated and memory T-cells.

Experimental Guidance and Technical Tips

• For flow cytometry, consider using activated mouse T-cells (e.g., anti-CD3/CD28 stimulated splenocytes) to ensure detectable ICOS expression.
• Titrate the antibody concentration to optimize the signal-to-noise ratio for your specific assay conditions.
• As a directly conjugated antibody, no secondary detection reagent is required, simplifying multicolor panel design.
• The immunogen corresponds to the extracellular domain (aa 21–142) of mouse ICOS, suggesting this antibody likely recognizes an N-terminal epitope.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with comprehensive access to IVD raw materials, including this ICOS/CD278 monoclonal antibody for immune function studies. Beyond this product, we offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to support your assay development needs. From early-stage concept to clinical application, our one-stop sourcing simplifies procurement for T-cell biology and other research areas.