Rabbit anti-Mouse I-A Monoclonal Antibody for FC - P14483

Core Product Specifications and Parameters

Biological Background: H-2 class II histocompatibility antigen, A beta chain Function and Localization

• Encoded by the H2-Ab1 gene in mouse, it is the beta chain of the H-2 class II histocompatibility antigen I-A.
• Functions as an MHC class II molecule, integral to adaptive immunity (UniProt keywords: Adaptive immunity, Immunity, MHC II).
• Localized to the cell membrane; it is a single-pass type I membrane protein with a transmembrane helix.
• The protein contains a signal peptide, suggesting it is processed through the secretory pathway.
• Predicted to undergo post-translational modifications including N-linked glycosylation (glycoprotein keyword), disulfide bond formation, and ubiquitin-like (Ubl) conjugation.
• Molecular weight of the unmodified precursor is approximately 30 kDa (UniProt: 30128 Da), consistent with the observed 30 kDa band.

Experimental Guidance and Technical Tips

• The immunogen (recombinant fusion protein covering amino acids 30-219, the extracellular domain) suggests the antibody targets the extracellular portion, making it suitable for flow cytometry detection of cell surface I-A on mouse cells.
• For FC applications, titrate the antibody and include appropriate isotype controls and viability dyes to ensure optimal staining.
• As the antibody is directly conjugated to , avoid multiplexing with other fluorophores that emit in the same channel (e.g., FITC, GFP) to prevent signal overlap.
• This product is validated for use with mouse samples; cross-reactivity with other species has not been determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for IVD raw materials, supporting diagnostic manufacturers and research laboratories with validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-mouse I-A monoclonal antibody addresses immunology research needs, particularly those centered on MHC class II biology and adaptive immunity. For specialized or rare-target raw-material procurement, CamelBio provides end-to-end supply chain assistance, connecting your projects with the necessary reagents from concept to clinic.