Rabbit anti-Mouse I-A Monoclonal Antibody for FC - P14483

Core Product Specifications and Parameters

Biological Background: I-A (H2-Ab1) Function and Localization

• Encoded by the H2-Ab1 gene, the I-A beta chain pairs with an alpha chain to form the MHC class II heterodimer, a critical component of adaptive immunity.
• MHC class II molecules present processed extracellular antigens to CD4+ helper T cells, initiating immune responses.
• Subcellularly, it resides on the cell membrane as a single-pass transmembrane protein, with its extracellular domain exposed for antigen presentation.
• The protein features a signal peptide, disulfide bonds in the extracellular region, and a cytoplasmic tail subject to ubiquitination.
• Multiple post-translational modifications include glycosylation and disulfide bond formation, typical of MHC class II glycoproteins.
• Belongs to the MHC class II family, with known three-dimensional structures deposited in the PDB (keyword: 3D-structure).
• Keyword annotations highlight its role in immunity, adaptive immunity, and its membrane localization.

Experimental Guidance and Technical Tips

• Designed for flow cytometry (FC); the conjugate provides bright, photostable fluorescence suitable for multicolor panels.
• The immunogen covers amino acids 30–219 of the extracellular domain, suggesting recognition of the native conformation—validate in relevant mouse cell lines or primary cells.
• Titrate the antibody to optimize the signal-to-noise ratio for your specific sample type (e.g., splenocytes, lymph node cells).
• When analyzing mouse I-A expression, consider co-staining with lineage markers to gate on specific immune cell populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated monoclonal and polyclonal antibodies, optimized antigen pairs, bulk ancillary reagents, and rare target raw materials. For mouse MHC class II research, this antibody serves as a reliable tool in immunology studies, and CamelBio can support you with tailored procurement solutions from concept to clinic.