Rabbit anti-Mouse H-2Kd/H-2Dd Monoclonal Antibody for FC - P01902 / P01900

Core Product Specifications and Parameters

Biological Background: H-2Kd/H-2Dd Function and Localization

• H-2 class I histocompatibility antigen, K-D alpha chain (encoded by H2-K1 gene in mouse), is a classical MHC class I molecule that presents peptide antigens to CD8+ T cells, playing a central role in adaptive immunity.
• This antibody recognizes both the H-2Kd and H-2Dd allotypes, which are polymorphic variants of MHC class I molecules expressed in certain mouse strains (e.g., BALB/c).
• As a type I transmembrane glycoprotein, it is anchored in the cell membrane via a single transmembrane helix and possesses an extracellular peptide-binding groove formed by α1 and α2 domains, with a supporting α3 and β2-microglobulin domain.
• The molecule is characterized by conserved structural features including an N-terminal signal peptide for ER targeting, intrachain disulfide bonds, and N-linked glycosylation, with post-translational modifications critical for stability and peptide loading.
• Subcellular localization: predominantly expressed on the plasma membrane, but also cycles through endosomal compartments as part of antigen processing and presentation pathways.
• Keywords: 3D-structure, Direct protein sequencing, Disulfide bond, Glycoprotein, Immunity, Membrane, MHC I, Phosphoprotein, Reference proteome, Signal, Transmembrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• For flow cytometry, use this ABflo 488 conjugate in standard staining buffers; optimal dilution should be empirically determined by titration in the experimental cell system.
• Since the antibody targets both H-2Kd and H-2Dd, it may not discriminate between these two allotypes; verify specificity with appropriate knockout or allele-specific controls if needed.
• Perform Fc receptor blocking prior to staining to reduce non-specific binding, especially when using cells with high FcγR expression.
• ABflo 488 has spectral properties similar to FITC (Ex/Em ≈ 490/515 nm); ensure proper compensation when multiplexing with other fluorophores.
• Store the antibody at 4°C protected from light; avoid freeze-thaw cycles to maintain conjugate stability.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material supplier, CamelBio offers comprehensive one-stop sourcing for diagnostic manufacturers, including validated antibody pairs, optimized monoclonals, and tailored bulk reagents to accelerate your immunoassay development. This product targets mouse MHC class I molecules, supporting research in transplant immunology, vaccine development, and autoimmune disease—just one example of our capability to deliver critical biomaterials from discovery to clinic.