Rabbit anti-Mouse FOXP3 mAb for FC (intra) - Q99JB6

Core Product Specifications and Parameters

Biological Background: FOXP3 Function and Localization

• FOXP3 is a transcriptional regulator essential for the development and suppressive function of regulatory T-cells (Treg), critical for immune homeostasis. Related references: PMID:22813742
• It can act as either a transcriptional repressor or activator, coordinating with histone acetylases and deacetylases to modulate gene expression.
• FOXP3 suppresses cytokines by repressing IL2 and IFNG expression through interactions with transcription factors like RELA, NFATC2, RUNX1, and IKZF4. Related references: PMID:15790681 PMID:17377532 PMID:19696312
• It also activates Treg-associated genes like CTLA4, TNFRSF18, and IL2RA, partly via RUNX1. Related references: PMID:17377532
• FOXP3 inhibits Th17 cell differentiation by antagonizing RORC, reducing IL17 production. Related references: PMID:18368049
• Subcellularly, it is found in both the nucleus and cytoplasm.
• Foxp3 shows high expression in thymus and spleen, consistent with its role in immune regulation.

Experimental Guidance and Technical Tips

• This antibody is raised against a synthetic peptide corresponding to the N-terminal region (amino acids 1-100) of mouse Foxp3.
• For intracellular flow cytometry (FC intra), consider appropriate fixation and permeabilization protocols to enable access to the nuclear/cytoplasmic FOXP3 protein.
• Titrate the antibody to determine the optimal working concentration for your specific assay and cell type.
• Validate staining specificity in known Treg populations (e.g., CD4+CD25+ cells) or Foxp3-expressing cell lines.
• Cross-reactivity with human or other species has not been characterized; use on mouse samples is supported.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including high-quality monoclonal antibodies like this anti-FOXP3 antibody for immune regulation and autoimmune disease research. Our supply chain covers validated antibody pairs, optimized bulk reagents, and rare target sourcing, empowering your diagnostic development from concept to clinic.