Rabbit anti-Mouse Flt3/CD135 Monoclonal Antibody for FC - Q00342

Core Product Specifications and Parameters

Biological Background: Flt3/CD135 Function and Localization

• Receptor-type tyrosine-protein kinase FLT3 (also known as CD135, FLK-2, fetal liver kinase 2) acts as a cell-surface receptor for the cytokine FLT3LG, regulating differentiation, proliferation and survival of hematopoietic progenitor cells and dendritic cells.
• Upon ligand binding, FLT3 promotes phosphorylation of SHC1, AKT1 and activation of MTOR, as well as RAS-dependent signaling via MAPK1/ERK2 and MAPK3/ERK1. It also mediates phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A/STAT5B, though wild-type FLT3 activation of STAT5 is marginal.
• Mutations causing constitutive kinase activity drive cell proliferation and apoptosis resistance through multiple downstream pathways, highlighting its proto-oncogenic role.
• Tissue expression is predominantly in hematopoietic stem and progenitor cell-enriched populations, with additional detection in brain, placenta and testis.
• Subcellularly, FLT3 localizes to the plasma membrane and the endoplasmic reticulum lumen.
• The protein features extracellular immunoglobulin domains, a single transmembrane helix, and an intracellular tyrosine kinase domain. Key post-translational modifications include glycosylation, disulfide bond formation, and ligand-induced autophosphorylation.
• Keywords associated with FLT3: ATP-binding, Disulfide bond, Glycoprotein, Immunoglobulin domain, Kinase, Membrane, Nucleotide-binding, Phosphoprotein, Proto-oncogene, Receptor, Transferase, Transmembrane helix, Tyrosine-protein kinase, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant fusion protein spanning amino acids 28–544 of mouse CD135 (NP_034359.2). Consider verifying reactivity with the native protein on primary mouse hematopoietic cells.
• For flow cytometry, titrate the antibody on mouse spleen or bone marrow cells to determine the optimal staining concentration. Use Fc receptor blocking to minimize non-specific binding.
• When designing multicolor panels, account for the fluorophore’s spectral properties and perform appropriate compensation controls.
• The antibody is recommended for mouse samples; cross-reactivity with other species has not been reported and should be tested independently if required.
• Validate specificity by comparing staining in Flt3-knockout models or with cell lines differentially expressing CD135.
• As FLT3 functions in hematopoietic stem cell maintenance and dendritic cell development, this reagent can support immunophenotyping, leukemia stem cell detection, or investigation of FLT3-ITD-driven signaling.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to support diagnostic manufacturers and research laboratories. Through strategic sourcing, we can help secure rare-target antibodies and recombinant proteins, such as anti-Flt3/CD135 reagents for flow cytometry applications. Our supply capabilities extend to custom assay development and technical consultation, ensuring seamless integration from concept to clinical implementation in hematopoietic cell and oncology research.