Rabbit anti-Mouse F4/80 Monoclonal Antibody for Flow Cytometry - Q61549

Core Product Specifications and Parameters

Biological Background: F4/80 Function and Localization

• F4/80, also known as Adhesion G protein-coupled receptor E1 (ADGRE1) or EMR1, is a cell surface glycoprotein encoded by the Adgre1 gene in mice (Gene ID 13733) and the corresponding human gene EMR1 (Gene ID 2015).
• It belongs to the adhesion GPCR family, characterized by a large extracellular domain containing EGF-like repeats and a mucin-like stalk, and is involved in cell adhesion and immune cell interactions.
• F4/80 is specifically expressed on the surface of macrophages, but is absent from macrophages located in T-cell areas of lymph nodes and spleen, and shows low expression on blood monocytes, making it a pan-macrophage marker.
• The protein may play a role in the development and function of regulatory T-cells (Tregs), indicating a function in adaptive immunity.
• Subcellular localization is at the cell membrane, consistent with its role as a transmembrane receptor involved in cell-cell interactions and signaling.
• The murine F4/80 protein has a predicted molecular weight of approximately 102 kDa, matching the observed protein weight of 102 kDa.
• Keywords from UniProt include Adaptive immunity, Cell membrane, Disulfide bond, EGF-like domain, G protein-coupled receptor, Glycoprotein, Immunity, Membrane, Receptor, Reference proteome, Repeat, Signal, Transducer, Transmembrane, Transmembrane helix, highlighting its structural features and immune function.

Experimental Guidance and Technical Tips

• This -conjugated rabbit monoclonal antibody is suitable for direct flow cytometry staining of mouse F4/80, enabling identification of macrophage populations without the need for a secondary antibody.
• The immunogen used comprises amino acids 28-644 of mouse F4/80, covering the extracellular domain, which may contribute to specific recognition of native cell surface F4/80.
• When designing flow cytometry panels, consider combining with other macrophage markers (e.g., CD11b) and lineage markers to discriminate macrophage subsets effectively.
• Validation of staining should be performed in the relevant mouse sample system, using appropriate controls such as isotype controls and positive/negative cell populations.
• The predicted molecular weight of the target is ~102 kDa; however, confirmation via Western blot may require appropriate denaturing conditions due to its highly glycosylated nature.

CamelBio: Your One-Stop Sourcing Bridge

As your dedicated one-stop IVD raw-material partner, CamelBio offers a comprehensive portfolio including this anti-F4/80 antibody and related macrophage markers, along with antibody pairs, bulk reagents, and customized sourcing solutions. From early development to clinical application, our expert team helps streamline your supply chain for diagnostic and research needs.