Rabbit anti-Mouse E-Cadherin/CD324 Monoclonal Antibody for FC - P09803

Core Product Specifications and Parameters

Biological Background: E-Cadherin Function and Localization

• Cadherin-1, also known as E-cadherin, uvomorulin, or ARC-1, is a member of the cadherin family of calcium-dependent cell adhesion proteins.
• It mediates homophilic cell–cell adhesion, playing a key role in sorting heterogeneous cell types. Related references: PMID:11976333
• CDH1 regulates cell–cell adhesions, mobility, and proliferation of epithelial cells. Related references: PMID:11976333
• The protein promotes desmosome formation by facilitating recruitment of DSG2 and DSP to cell junctions.
• E-cadherin acts as a potent invasive suppressor and serves as a ligand for integrin alpha‑E/beta‑7.
• Subcellular localization includes the adherens junction, desmosome, cell membrane, endosome, Golgi apparatus, and cytoplasm.
• Tissue expression is predominantly in non‑neural epithelial tissues, including the organ of Corti, granuloma macrophages, and epidermal keratinocytes.
• Keywords: Calcium, cell adhesion, cell junction, glycoprotein, phosphoprotein, Ubl conjugation, and cleavage on pair of basic residues.

Experimental Guidance and Technical Tips

• conjugation enables direct detection in flow cytometry without a secondary antibody; perform titrations to determine the optimal working concentration.
• The immunogen corresponds to amino acids 157–369 of the mouse E‑cadherin extracellular domain, so the antibody is expected to recognize the native surface protein on live cells.
• For intracellular detection, include appropriate permeabilization and blocking steps; validate in your specific sample system.
• Mouse cross‑reactivity is confirmed; testing on other species may require sequence alignment due to the conserved cadherin motifs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw‑material procurement for diagnostic developers and researchers. Beyond this anti‑E‑cadherin monoclonal antibody, we offer validated antibody pairs, bulk ancillary reagents, and sourcing support for rare targets in the cell adhesion and epithelial biology space. Let CamelBio be your reliable partner from concept to clinic.