Rabbit anti-Mouse E-Cadherin/CD324 Monoclonal Antibody for FC - P09803

Core Product Specifications and Parameters

Biological Background: E-Cadherin/CD324 Function and Localization

• Cadherin-1 (CDH1), also known as E-cadherin, epithelial cadherin (E-cad), uvomorulin (UM), or ARC-1, is a calcium-dependent cell adhesion protein essential for maintaining tissue architecture and epithelial integrity.
• E-cadherin mediates homophilic cell-cell adhesion, contributing to the sorting of heterogeneous cell types and regulating epithelial cell mobility and proliferation (PubMed:11976333). Related references: PMID:11976333
• Beyond adhesion, CDH1 promotes the organization of radial actin fibers and cellular response to contractile forces via interaction with AMOTL2, and facilitates the early formation of desmosome junctions by recruiting DSG2 and DSP.
• The protein acts as a potent invasion suppressor and serves as a ligand for integrin alpha-E/beta-7, highlighting its roles in tumor suppression and immune cell interactions.
• A C-terminal fragment (E-Cad/CTF2) functions in non-amyloidogenic degradation of amyloid-beta precursors, potentially linking E-cadherin to Alzheimer’s disease pathways.
• Notably, mouse E-cadherin does not natively act as a receptor for Listeria monocytogenes internalin A; however, a single surface residue mutation can confer receptor activity, underscoring structural specificity.
• Subcellularly, E-cadherin is predominantly localized at adherens junctions and the cell membrane, but also detected in endosomes, the trans-Golgi network, cytoplasm, and desmosomes.
• Tissue expression is broad in non-neural epithelia and includes specific sites such as inner and outer pillar cells of the organ of Corti (PubMed:30639848), granuloma macrophages (PubMed:27760340), and epidermal keratinocytes (PubMed:18079750, PubMed:27375112, PubMed:30660666). Related references: PMID:30639848 PMID:27760340 PMID:18079750 PMID:27375112 PMID:30660666

Experimental Guidance and Technical Tips

• The immunogen (amino acids 157–369 of mouse E-Cadherin/CD324) covers an extracellular domain region, likely ensuring antibody accessibility in live-cell flow cytometry applications.
• For flow cytometry (FC), consider using single-cell suspensions from mouse tissues or cell lines known to express E-cadherin; include appropriate isotype controls and live/dead staining for accurate gating.
• Since the antibody is conjugated with , avoid fluorophore bleaching by minimizing light exposure and optimizing laser settings.
• Validate antibody performance in your specific experimental system by titrating the antibody and including positive and negative control populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a reliable one-stop sourcing partner for diagnostic manufacturers and research laboratories, offering a comprehensive portfolio of IVD raw materials including validated antibody pairs, monoclonal antibodies, and ancillary reagents. The E-cadherin/CD324 antibody supports research in epithelial cell biology, cancer metastasis, and cell adhesion studies. We specialize in bridging supply gaps for rare-target reagents and bulk-order requirements, ensuring seamless procurement from concept to clinic.