Rabbit anti-Mouse CXCR2/IL-8RB Monoclonal Antibody for FC - P35343

Core Product Specifications and Parameters

Biological Background: CXCR2/IL-8RB Function and Localization

• C-X-C chemokine receptor type 2 (CXCR2), also known as GRO/MGSA receptor or IL-8 receptor B (IL-8RB), is encoded by the Cxcr2 gene in mouse (aliases: Cmkar2, Gpcr16, Il8rb).
• CXCR2 serves as a high-affinity receptor for interleukin-8 (IL-8) and related chemokines (e.g., CXCL3, GRO/MGSA, NAP-2). Ligand binding triggers G protein-mediated activation of the phosphatidylinositol-calcium second messenger system, leading to neutrophil activation and chemotaxis.
• In vivo, CXCR2 is involved in homeostatic wound healing, guiding neutrophil migration to necrotic tissue while avoiding collateral damage to healthy areas. This chemotactic signaling is essential for intravascular neutrophil recruitment to injury sites. Related references: PMID:20947763
• As a classical G protein-coupled receptor (GPCR), CXCR2 possesses seven transmembrane domains and transduces extracellular signals via heterotrimeric G proteins. It belongs to the chemokine receptor subfamily within the rhodopsin-like receptor class.
• CXCR2 localizes to the cell membrane. Post-translational modifications include N-linked glycosylation, phosphorylation, and disulfide bond formation, which are critical for receptor stability and signaling function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (aa 1–44). For flow cytometry, this antibody may bind native CXCR2 on live cells; consider titrating and using appropriate isotype controls. Validate specificity with CXCR2-knockout or -knockdown cell models or competitive blocking.
• As a rabbit monoclonal antibody, it typically offers high affinity and low lot-to-lot variability. In multicolor flow cytometry, verify fluorophore brightness and compensation. is compatible with FITC/GFP channels.
• Sample preparation: use freshly isolated mouse neutrophils or CXCR2-expressing cell lines. Avoid prolonged fixation, as it may alter epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive raw-material solutions for IVD developers and researchers, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. This anti-mouse CXCR2 antibody supports immunology and inflammation research, and CamelBio can assist with scaling supply, technical consultation, and integration into diagnostic workflows. With a focus on bridging innovation to clinic, we aim to be your trusted partner from concept to market.