Rabbit anti-Mouse CD98 Monoclonal Antibody for Flow Cytometry - P10852

Core Product Specifications and Parameters

Biological Background: CD98 Function and Localization

• CD98, also known as 4F2 cell-surface antigen heavy chain (4F2hc) or SLC3A2, is encoded by the Slc3a2 gene. It acts as a chaperone for SLC7 family amino acid transporters, facilitating their correct biogenesis and trafficking to the plasma membrane.
  Related references: PMID:10574970, PMID:10734121, PMID:11011012
• SLC3A2 forms heterodimers with multiple SLC7 light chains (e.g., SLC7A5, SLC7A11), mediating the exchange of neutral, dibasic, and anionic amino acids across the membrane; substrate specificity is determined by the SLC7A partner.
• CD98 localizes to the apical and basolateral plasma membrane, lysosomal membrane, melanosomes, and cell junctions, consistent with its roles in transport and cell adhesion.
• Ubiquitously expressed, with highest levels in kidney, small intestine, spleen, thymus, and liver; detected on embryonic epithelial cells and brain capillary endothelia.
  Related references: PMID:9915839
• The protein undergoes post-translational modifications including glycosylation, phosphorylation, disulfide bond formation, and ubiquitination, as indicated by UniProt keywords.
• Beyond transport, CD98 modulates integrin signaling, cell spreading, migration, and tumor progression; it also contributes to mTORC1 activation by facilitating lysosomal leucine uptake via the SLC7A5-LAPTM4B complex.

Experimental Guidance and Technical Tips

• The antibody is raised against a recombinant protein spanning amino acids 105-526 of mouse CD98, which includes the extracellular domain; this may enable recognition of native surface-expressed CD98 in flow cytometry.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your cell type and experimental conditions; include appropriate isotype controls and Fc blocking reagents to minimize non-specific binding.
• Validate antibody reactivity with CD98-positive and negative cell populations (e.g., lymphocytes vs. erythrocytes) to confirm specificity.
• Because CD98 is expressed on many proliferating cells, consider the impact of activation status or cell cycle on staining intensity.

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