Rabbit anti-Mouse CD98 Monoclonal Antibody for Flow Cytometry - P10852

Core Product Specifications and Parameters

Biological Background: CD98 (SLC3A2) Function and Localization

• CD98 heavy chain (SLC3A2, 4F2hc) is a type II transmembrane glycoprotein that acts as a molecular chaperone, facilitating the biogenesis, plasma membrane trafficking, and stability of heterodimeric amino acid transporters [PMID:10574970, PMID:10734121, PMID:11011012]. Related references: PMID:10574970 PMID:10734121 PMID:11011012

• It forms covalent heterodimers via a disulfide bond with various SLC7 light chains, creating functional amino acid exchangers with distinct substrate specificities: system L (large neutral amino acids), system xc− (cystine/glutamate), and system y+L (dibasic amino acids) [PMID:10574970, PMID:10734121, PMID:11011012]. Related references: PMID:10574970 PMID:10734121 PMID:11011012

• Resides primarily at the plasma membrane (both apical and basolateral domains), but also localizes to lysosome membranes, melanosomes, and cell junctions, consistent with its roles in transport and cell adhesion.

• Undergoes extensive N‑glycosylation and phosphorylation; contains conserved disulfide bonds critical for its structure and interaction with light chains.

• Widely expressed in mouse tissues, with highest levels in kidney, small intestine, spleen, thymus, and liver; also detected on brain capillary endothelial cells and embryonic epithelia [PMID:9915839]. Related references: PMID:9915839

• Beyond transport, CD98 modulates integrin‑dependent signaling pathways, promoting cell spreading, migration, and tumor progression (by similarity).

Experimental Guidance and Technical Tips

• For flow cytometry, use standard surface staining protocols with live cells; no permeabilization required as CD98 is a cell surface antigen.
• Validate antibody performance in the relevant mouse cell type or tissue, taking into account its broad expression; use appropriate isotype controls and Fc block to minimize background.
• As a directly conjugated antibody, minimize light exposure and use fresh aliquots to maintain fluorescence intensity.
• Consider co‑staining with cell type‑specific markers to distinguish CD98‑positive populations.
• Due to potential internalization of CD98, incubations should be at 4°C or on ice to prevent antibody uptake.

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CamelBio empowers diagnostic developers with one-stop access to high-quality IVD raw materials, including ready-to-use conjugated antibodies like this anti‑CD98 () for immunology and transporter research. Our portfolio spans validated monoclonal/polyclonal antibodies, matched antibody pairs, and ancillary reagents, backed by technical expertise to accelerate your assay development from concept to clinic.