Rabbit Anti-Mouse CD97 Monoclonal Antibody for Flow Cytometry - Q9Z0M6

Core Product Specifications and Parameters

Biological Background: CD97 (Adgre5) Function and Localization

• CD97, also designated Adhesion G protein-coupled receptor E5 (ADGRE5), is a member of the adhesion GPCR subfamily and contains multiple EGF-like domains in its extracellular region.
• It functions in both cell adhesion and intracellular signaling, and plays a critical role in leukocyte migration, particularly during early immune responses.
• CD97 is primarily localized to the cell membrane, but can also be cleaved and secreted into the extracellular space.
• The protein is widely expressed, with highest levels in the lung and thymus; in the spleen, it is found on most myeloid cells and subsets of T, B, and NK cells.
• Post-translational modifications include N-linked glycosylation and phosphorylation, and the gene undergoes alternative splicing to generate multiple isoforms.
• Keywords associated with CD97 include alternative splicing, calcium binding, cell adhesion, EGF-like domain, G protein-coupled receptor, glycoprotein, and phosphoprotein.

Experimental Guidance and Technical Tips

• This antibody recognizes an epitope within the extracellular domain (amino acids 24–265) of mouse CD97, making it suitable for detecting surface CD97 in flow cytometry applications.
• As an conjugate, it excites at approximately 590 nm with emission around 617 nm; verify compatibility with your instrument’s laser and filter configuration.
• Titrate the antibody to determine the optimal concentration for your specific cell type and avoid non-specific binding by including appropriate isotype controls.
• Given CD97 expression on various leukocyte subsets, employ gating strategies to identify populations of interest and validate staining with known positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing bridge for IVD raw materials, connecting diagnostic manufacturers, laboratories, and research institutes with validated reagents from concept to clinic. This anti-mouse CD97 monoclonal antibody, developed for flow cytometry, is part of our comprehensive portfolio for immunology and leukocyte biology research. In addition to optimized antibodies, we supply validated antibody pairs, bulk ancillary reagents, and rare-target raw materials to support your assay development pipeline.