Rabbit anti-Mouse CD97 Monoclonal Antibody for Flow Cytometry - Q9Z0M6

Core Product Specifications and Parameters

Biological Background: Adhesion G protein-coupled receptor E5 (CD97) Function and Localization

• CD97, also known as Adhesion G protein-coupled receptor E5 or leukocyte antigen CD97, is a member of the adhesion G protein-coupled receptor (GPCR) family, characterized by an extended N-terminal extracellular domain containing EGF-like repeats.
• The encoded receptor is a glycoprotein that undergoes proteolytic processing and alternative splicing, giving rise to multiple isoforms with varying extracellular region lengths.
• CD97 functions as a dual adhesion and signaling receptor early after leukocyte activation, playing an essential role in leukocyte migration.
• The protein localizes primarily to the cell membrane but can also be found secreted into the extracellular space.
• In mouse tissues, CD97 is expressed ubiquitously but at particularly high levels in the lung and thymus; within the spleen, it is detected on most myeloid cells and variable portions of T-cells, B-cells, and NK cells, while bone marrow expression is found in nearly all myeloid cells and is largely absent from erythroid cells.
• Key post-translational modifications include glycosylation, disulfide bond formation, and phosphorylation, with calcium binding modulated by EGF-like domains.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal extracellular region (residues 24–265) of mouse CD97, suggesting the antibody is likely to recognize cell surface CD97 on viable cells, making it suitable for flow cytometry applications.
• Because CD97 is expressed on leukocyte populations, validating this antibody in mouse spleenocytes or bone marrow cells can help confirm detection of myeloid and lymphoid subsets.
• The conjugate enables direct one-step staining without a secondary antibody, minimizing background; however, appropriate Fc receptor blocking and optimal titration should be determined empirically.
• Given the protein's molecular weight of ~90 kDa, verify that the staining pattern aligns with anticipated cell surface expression profiles in test samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers investigating leukocyte adhesion and migration, we offer a range of validated primary antibodies, including this anti-CD97 clone, as well as bulk ancillary reagents and support for sourcing rare target reagents. Rely on CamelBio to streamline your raw-material supply chain and accelerate your IVD development.