Rabbit anti-Mouse CD95/FAS Monoclonal Antibody for Flow Cytometry - P25446

Core Product Specifications and Parameters

Biological Background: CD95/FAS Function and Localization

• CD95 (also known as FAS, Apo-1, or TNFR6) is a member of the tumor necrosis factor receptor superfamily and functions as the receptor for FASLG/TNFSF6.
• Upon ligand binding, CD95 recruits FADD and caspase-8 to form the death-inducing signaling complex (DISC), triggering the caspase cascade that mediates apoptosis (By similarity).
• FAS-mediated apoptosis plays a critical role in peripheral tolerance and the antigen-stimulated suicide of mature T-cells, thereby regulating immune homeostasis.
• The protein is localized at the cell membrane and is enriched in membrane rafts, which are microdomains important for signal transduction.
• CD95 is expressed in multiple tissues, including thymus, liver, lung, heart, and adult ovary, indicating broad physiological roles.
• Post-translational modifications include palmitoylation, phosphorylation, and N-linked glycosylation; it also contains disulfide bonds and a signal peptide.
• The gene is designated Fas (also known as Apt1, Tnfrsf6 in mouse), corresponding to human GeneID 355 and mouse GeneID 14102.

Experimental Guidance and Technical Tips

• The immunogen is recombinant mouse CD95/FAS protein; the antibody likely recognizes an epitope preserved in the native conformation, making it suitable for surface staining in flow cytometry.
• When performing flow cytometry, titrate the antibody to determine the optimal staining concentration for your specific cell type and assay conditions.
• This antibody is conjugated to ; store protected from light and avoid repeated freeze-thaw cycles to maintain fluorophore stability.
• CD95 expression can be induced or context-dependent; verify target expression on your cells of interest using a positive control such as mouse thymocytes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes key targets in apoptosis and immune research, such as this validated monoclonal antibody against CD95/FAS, which we can supply in custom conjugations and bulk formats. We support your workflow with reliable sourcing and expert technical guidance.