Rabbit anti-Mouse CD94 mAb for FC - O54707

Core Product Specifications and Parameters

Biological Background: CD94 Function and Localization

• CD94, also known as Killer cell lectin-like receptor subfamily D member 1 (KLRD1), is a transmembrane protein that belongs to the C-type lectin family. It serves as a subunit for both inhibitory and activating immune receptors on natural killer (NK) cells and some T cell subsets.
• In complex with KLRC1, CD94 forms an inhibitory receptor that recognizes non-classical MHC class Ib molecule MHC-E loaded with self-peptides. This interaction enables NK cells to monitor MHC class I expression, promoting self-tolerance and preventing autoimmunity.
• In complex with KLRC2, CD94 forms an activating receptor that also binds MHC-E, but this interaction triggers activating signals via TYROBP/DAP12, playing roles in adaptive NK cell responses and maternal-fetal tolerance.
• CD94 is primarily a ligand-binding subunit; it lacks intrinsic signaling capacity. Signaling is mediated through its partner chains—ITIM motifs in KLRC1 recruit phosphatases for inhibition, while KLRC2 associates with DAP12 for activation.
• Subcellularly, CD94 is localized to the cell membrane as a signal-anchor type II transmembrane protein. It contains a C-type lectin-like domain in the extracellular region.
• Post-translational modifications include disulfide bond formation and glycosylation, as indicated by UniProt keywords. The protein has a calculated molecular weight of approximately 20.8 kDa.
• CD94 is classified under keywords such as adaptive immunity, innate immunity, lectin, receptor, and transmembrane helix, highlighting its dual role in immune surveillance.

Experimental Guidance and Technical Tips

• The antibody is conjugated to , a bright fluorophore suitable for flow cytometry. Consider titrating the antibody (e.g., 0.5-5 µg per test) to determine the optimal staining concentration for your cell type.
• As the immunogen corresponds to the extracellular domain (aa 59-179), this antibody likely recognizes an epitope in the lectin-like domain, accessible on live cells without permeabilization.
• Validate antibody specificity by using CD94-transfected versus non-transfected cells, or by comparing staining on wild-type and CD94-knockout NK cells.
• For multicolor flow panels, check that does not overlap significantly with other fluorophores in the same channel (e.g., FITC, Alexa Fluor 488). Use appropriate compensation controls.
• This antibody is validated for mouse samples; for other species, cross-reactivity should be tested experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. For CD94-related NK cell and immune checkpoint studies, we offer validated antibody pairs, recombinant proteins, and bulk ancillary reagents. Explore our catalog for optimized monoclonal antibodies and custom sourcing solutions for rare targets.