Rabbit anti-Mouse CD90.2 Monoclonal Antibody for Flow Cytometry - P01831

Core Product Specifications and Parameters

Biological Background: Thy-1 Membrane Glycoprotein Function and Localization

• The Thy-1 membrane glycoprotein, also known as CD90, is encoded by the Thy1 gene in mouse. It is a member of the immunoglobulin superfamily, characterized by a single V-type immunoglobulin domain.
• Thy-1 is a glycosylphosphatidylinositol (GPI)-anchored protein localized to the outer leaflet of the cell membrane, as confirmed by direct protein sequencing.
• The protein is heavily N-glycosylated, with a predicted core molecular weight of approximately 18 kDa; however, glycans increase the apparent mass observed in experiments.
• Thy-1 is involved in cell-cell and cell-matrix interactions, particularly playing a role in synaptogenesis and neuronal development in the brain.
• This antibody specifically recognizes the Thy-1.2 (CD90.2) allelic form, a common allotype found in many mouse strains such as BALB/c and C57BL/6.
• Key structural features include an intramolecular disulfide bond and a mature polypeptide that begins with a pyroglutamic acid residue.

Experimental Guidance and Technical Tips

• This antibody is recommended for flow cytometry (FC) applications. The fluorophore offers bright, photostable fluorescence with excitation/emission peaks suitable for common red-orange channels.
• For optimal staining, consider titrating the antibody in the relevant experimental system to determine the ideal concentration. Always include appropriate isotype control and viability dyes.
• As the immunogen is a recombinant protein, the antibody recognizes a native epitope of mouse CD90.2. It is not expected to cross-react with human Thy-1 based on stated species reactivity.
• Avoid repeated freeze-thaw cycles and protect the conjugate from prolonged light exposure to maintain performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing ready-to-use IVD raw materials, including this Rabbit anti-Mouse CD90.2 monoclonal antibody. With a focus on immunology and stem cell marker targets like Thy-1, CamelBio streamlines procurement of validated antibodies, ancillary reagents, and custom sourcing for assay development and clinical translation needs.