Rabbit anti-Mouse CD90.2 Monoclonal Antibody for Flow Cytometry - P01831

Core Product Specifications and Parameters

Biological Background: Thy-1 Membrane Glycoprotein (CD90.2) Function and Localization

• Thy-1 membrane glycoprotein (CD90) is a GPI-anchored cell surface protein containing an immunoglobulin domain.
• Also known as Thy-1 antigen and T25, it is expressed on the cell membrane of various cell types.
• In mice, two alleles exist: Thy-1.1 and Thy-1.2 (CD90.2); this antibody specifically recognizes the Thy-1.2 variant.
• May participate in cell-cell and cell-ligand interactions during synaptogenesis and other processes in the brain.
• Post-translational modifications include glycosylation, disulfide bond formation, and pyroglutamic acid formation.
• The calculated molecular weight is approximately 18 kDa (18,080 Da); glycosylation increases the observed molecular weight.

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to , eliminating the need for a secondary antibody in flow cytometry experiments.
• Since the immunogen covers the extracellular domain, the antibody likely binds an extracellular epitope, making it suitable for live-cell staining.
• Validate in the appropriate mouse strain (expressing Thy-1.2) and include proper isotype controls.
• Titrate the antibody on positive cells (e.g., mouse thymocytes) to determine the optimal concentration for your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material procurement, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing for diagnostic and research laboratories. With this Rabbit anti-Mouse CD90.2 antibody, we support flow cytometry-based applications in immunophenotyping and neuroscience research. CamelBio streamlines your supply chain from concept to clinic.