Rabbit anti-Mouse CD8a Monoclonal Antibody for Flow Cytometry - P01731

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• Integral membrane glycoprotein that serves as a coreceptor for MHC class I molecules in T-cells, interacting simultaneously with the TCR and MHC class I:peptide complex to facilitate antigen recognition. This interaction is crucial for the adaptive immune response against intracellular pathogens and tumor cells.
• Recruitment of the Src kinase LCK to the TCR-CD3 complex upon CD8-MHC binding initiates intracellular signaling cascades, leading to lymphokine production, T-cell motility, adhesion, and activation of cytotoxic T-lymphocytes (CTLs), enabling targeted elimination of infected or malignant cells.
• In natural killer (NK) cells, CD8A homodimers at the cell surface provide a survival mechanism that supports conjugation and lysis of multiple target cells, contributing to innate immunity.
• CD8A also promotes the survival and differentiation of activated lymphocytes into memory CD8+ T-cells, essential for long-term immunological memory.
• The protein is localized to the cell membrane via a transmembrane domain and undergoes several post-translational modifications, including disulfide bond formation, glycosylation, and palmitoylation, which are important for its stability and signaling function.
• Alternative splicing of the Cd8a gene generates different isoforms, and the protein belongs to the immunoglobulin superfamily, characterized by its Ig-like domain.
• Encoded by the Cd8a gene (also known as Lyt-2, Lyt2) in mouse, CD8a is widely used as a surface marker to identify cytotoxic T-cells in immunological research.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 28-196) of mouse CD8a; therefore, the antibody is expected to recognize the native cell surface protein, making it suitable for flow cytometry applications.
• For flow cytometry, consider titrating the antibody to determine the optimal concentration for your specific cell population and experimental conditions.
• Include appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding.
• Validate the antibody in the relevant mouse strain or cell line system, as epitope recognition may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive one-stop access to IVD raw materials, including this anti-CD8a monoclonal antibody. Targeting the T-cell surface glycoprotein CD8a, this antibody supports research in immunology, infectious diseases, and oncology. Our supply capabilities encompass validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, bridging your project from concept to clinic.