Rabbit anti-Mouse CD8a Monoclonal Antibody for FC - P01731

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• CD8a is a T-cell surface glycoprotein serving as a coreceptor for MHC class I molecules; it engages the TCR-MHC I complex to initiate T-cell activation.
• Interaction with MHC I recruits the Src kinase LCK, which phosphorylates intracellular substrates, leading to lymphokine production, CTL activation, and target cell elimination.
• In NK cells, CD8A homodimers facilitate survival and serial killing by promoting conjugation and lysis of multiple target cells.
• The protein also contributes to the differentiation of activated lymphocytes into memory CD8 T-cells.
• CD8a contains an immunoglobulin domain and features post-translational modifications including disulfide bonds, glycosylation, and palmitoylation.
• The protein is a single-pass transmembrane glycoprotein with a signal peptide and is localized to the cell membrane; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell lines or primary samples, as staining intensity may vary.
• Validate antibody reactivity in the mouse model system of interest; cross-reactivity is reported only for mouse CD8a.
• Use appropriate isotype controls and compensation controls when using this -conjugated antibody to ensure accurate gating and interpretation.
• Consider that the immunogen corresponds to the extracellular domain (aa 28-196) of mouse CD8a, indicating the antibody likely recognizes an epitope in that region; verify if this epitope is preserved in your experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated monoclonal antibodies like this anti-mouse CD8a clone to support immune profiling and diagnostic development. Our portfolio includes a wide range of CD markers, antibody pairs for sandwich immunoassays, and ancillary reagents, enabling streamlined procurement from concept to clinical application. Whatever your CD8a-related research or assay needs, CamelBio provides tailored solutions and expert technical support to help you advance with confidence.