Rabbit anti-Mouse CD8a Monoclonal Antibody for FC - P01731

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• Member of the immunoglobulin superfamily, CD8a (T-cell surface glycoprotein CD8 alpha chain, also known as Lyt-2) is a coreceptor essential for adaptive immunity. The gene Cd8a undergoes alternative splicing to generate membrane-associated and secreted isoforms.
• Associates with MHC class I molecules on antigen-presenting cells, simultaneously interacting with the T-cell receptor (TCR) to enhance antigen recognition by cytotoxic T-lymphocytes (CTLs).
• Recruits the Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling cascades that drive lymphokine production, T-cell activation, adhesion, and target cell lysis.
• In natural killer (NK) cells, CD8a homodimers contribute to survival signaling and enable serial killing of multiple target cells.
• Functions beyond CTL-mediated cytotoxicity include promoting differentiation and long-term maintenance of memory CD8+ T-cells.
• Predominantly localized to the cell membrane via a single-pass transmembrane domain, with post-translational modifications including disulfide bond formation, N-linked glycosylation, and palmitoylation.
• Key structural features include an extracellular immunoglobulin V-like domain and a cytoplasmic tail that mediates signal transduction.

Experimental Guidance and Technical Tips

• This antibody is validated for flow cytometry (FC). For optimal performance, titrate the antibody in your specific experimental system, as optimal dilutions may vary with cell type and staining conditions.
• The immunogen spans the extracellular domain (aa 28–196), suggesting recognition of the mature, surface-expressed form of mouse CD8a. Consider its application in detecting CD8+ T-cells, CTLs, and NK subsets in mouse samples.
• Standard FC protocols with appropriate blocking (e.g., Fc receptor block) and viability dyes are recommended to minimize non-specific binding. Confirm cross-reactivity with your mouse strain, as polymorphisms may impact epitope accessibility.
• For multiplexed analyses, verify compatibility with other fluorophore-conjugated antibodies in your panel; the dye is designed for violet laser excitation (405 nm) and typically offers high brightness.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, supplying high-quality antibodies and reagents to diagnostic manufacturers and research laboratories worldwide. This anti-mouse CD8a monoclonal antibody aligns with immune monitoring and T-cell research needs, and we can further assist in sourcing matched antibody pairs, ancillary reagents, and bulk quantities to streamline your assay development. Reach out to explore customized monoclonal and polyclonal antibody solutions, flexible scale-up options, and consultation services—all designed to bridge your concept to clinic efficiently.