Rabbit anti-Mouse CD88/C5aR Monoclonal Antibody for Flow Cytometry - P30993

Core Product Specifications and Parameters

Biological Background: CD88/C5aR (C5AR1) Function and Localization

• C5a anaphylatoxin chemotactic receptor 1 (C5AR1, also known as CD88 or C5aR) is a G protein-coupled receptor for the inflammatory peptide C5a. Related references: PMID:37852260
• Upon C5a binding, C5AR1 triggers chemotaxis, granule enzyme release, intracellular calcium mobilization, and superoxide anion production, coupling to G(i)/G(o) proteins to inhibit adenylate cyclase. Related references: PMID:37852260
• The ligand interacts with a high-affinity site on the extracellular N-terminus (aa 1-37) and a second site in the transmembrane region that activates downstream signaling (By similarity).
• C5AR1 localizes to the cell membrane and cytoplasmic vesicles, consistent with its role as a transmembrane receptor.
• Post-translational modifications include glycosylation, phosphorylation, and sulfation; the receptor contains disulfide bonds and seven transmembrane helices typical of GPCRs.
• C5AR1 is involved in innate immunity, chemotaxis, and inflammatory responses, and the protein has been structurally characterized (3D-structure available).

Experimental Guidance and Technical Tips

• The monoclonal antibody was generated against a recombinant protein spanning amino acids 1-37 of the mouse C5AR1 extracellular N-terminus, a region critical for high-affinity C5a binding, enabling its use in flow cytometry to detect cell surface expression.
• For flow cytometry, consider titrating the conjugate to determine optimal staining concentration and include appropriate isotype control antibodies and Fc receptor blocking steps.
• Validation in mouse cell lines or primary immune cells (e.g., neutrophils, macrophages) known to express C5AR1 is recommended to confirm specificity.

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