Rabbit anti-Mouse CD88/C5aR mAb - P30993

Core Product Specifications and Parameters

Biological Background: C5aR1 Function and Localization

• C5aR1 (CD88) is a G protein-coupled receptor for the potent anaphylatoxin C5a, a key mediator of inflammatory and immune responses.
• Ligand binding stimulates chemotaxis, granule enzyme release, intracellular calcium mobilization, and superoxide anion production via G(i)/G(o) protein-mediated inhibition of adenylate cyclase. Related references: PMID:37852260
• The receptor is predominantly localized at the cell membrane and can also be found in cytoplasmic vesicles.
• C5aR1 contains seven transmembrane helices, conserved disulfide bonds, and undergoes post-translational modifications such as glycosylation, phosphorylation, and sulfation.
• The extracellular N-terminus provides a high-affinity binding site for C5a, while the transmembrane region is responsible for initiating downstream signaling.

Experimental Guidance and Technical Tips

• This conjugate is suitable for direct flow cytometry (FC) and targets the N-terminal extracellular domain (amino acids 1-37) of mouse C5aR1.
• For optimal staining, validate the antibody in the relevant mouse cell or tissue samples and include appropriate isotype controls and viability dyes.
• Given the GPCR nature of the target, consider assessing receptor surface expression under non-permeabilizing conditions and verify specificity using C5aR1 knockout or knockdown controls.
• As a monoclonal rabbit antibody, it typically offers high affinity and reproducibility; however, titration is recommended to determine the optimal working concentration for your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with one-stop access to validated IVD raw materials, including this high-quality anti-mouse C5aR1 antibody for inflammation and immunology research. We offer comprehensive supply solutions—from optimized monoclonal antibodies and bulk ancillary reagents to rare target sourcing—to streamline your assay development pipeline. Partner with CamelBio to bridge the gap from concept to clinic with reliable, scalable raw material support.