Rabbit anti-Mouse CD87 (uPAR) Monoclonal Antibody for Flow Cytometry - P35456

Core Product Specifications and Parameters

Biological Background: CD87 (uPAR) Function and Localization

• CD87, encoded by the Plaur gene, is the urokinase plasminogen activator surface receptor (uPAR), a glycosylphosphatidylinositol (GPI)-anchored glycoprotein.
• It serves as a high-affinity receptor for urokinase-type plasminogen activator (uPA), localizing and activating uPA at the cell surface to convert plasminogen into plasmin, thereby initiating pericellular proteolysis and extracellular matrix (ECM) remodeling.
• Beyond proteolysis, CD87 binds vitronectin, an ECM component, facilitating cell-matrix adhesion and indirectly modulating integrin-mediated signaling pathways.
• Through its dual interaction with uPA and vitronectin, CD87 coordinates dynamic changes in cell migration, proliferation, and tissue remodeling.
• Subcellularly, CD87 is localized to the cell membrane and is enriched at invadopodia, specialized membrane protrusions that mediate ECM degradation; a soluble, secreted form is also generated.
• At the protein level, CD87 is expressed in angiogenic endothelial cells, highlighting its role in vascular remodeling and angiogenesis.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 24-297), suggesting that the antibody should recognize cell surface CD87. Validate in relevant mouse cell lines or primary cells expressing CD87, such as angiogenic endothelial cells.
• For flow cytometry, titrate the conjugate to determine the optimal staining concentration. Use appropriate controls, including isotype controls and Fc receptor blocking, to minimize nonspecific binding.
• is a bright, far-red fluorophore compatible with standard flow cytometers. Protect the antibody from light and avoid repeated freeze-thaw cycles to maintain conjugate stability.
• Assess staining on live cells for surface detection; if intracellular staining is needed, a fixation/permeabilization step may be required. Confirm specificity by comparing signal in Plaur-knockout cells or using a blocking peptide, if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to validated IVD raw materials. This Rabbit anti-Mouse CD87 monoclonal antibody supports flow cytometry applications targeting the urokinase receptor (uPAR/CD87) in mouse samples. Beyond single reagents, CamelBio offers optimized monoclonal/polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your assay development from concept to clinic.