Rabbit anti-Mouse CD83 Monoclonal Antibody for Flow Cytometry - O88324

Core Product Specifications and Parameters

Biological Background: CD83 Function and Localization

• CD83 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily, characterized by an extracellular immunoglobulin domain, a single transmembrane helix, and a cytoplasmic tail.
• It is predominantly expressed on the surface of dendritic cells, lymphocytes, and other immune cells, with abundant expression in the spleen and brain, and detectable in most tissues.
• CD83 plays an essential role in CD4+ T-cell selection, differentiation, and stability by regulating the E3 ubiquitin ligase MARCHF8, which controls MHC II trafficking. Related references: PMID:27503071 PMID:27503069
• Additionally, CD83 inhibits MARCHF1 association with MHC II and ubiquitination of MHC II, further modulating antigen presentation.
• It acts as an important modulator of protective immune responses against acute infections. Related references: PMID:31527313
• The protein is subject to post-translational modifications including disulfide bond formation and glycosylation.
• CD83 is localized at the cell membrane.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• Include appropriate isotype controls to distinguish specific from non-specific binding.
• Since CD83 is a membrane protein, use live cell staining protocols or fix/permeabilize cells appropriately; note that fixation may affect the epitope recognized by this antibody.
• Validate the antibody in mouse spleen or lymphocyte samples where CD83 expression is expected to be high.
• The immunogen corresponds to the extracellular region (amino acids 22-133) of mouse CD83, suggesting the antibody likely binds an extracellular epitope; consider this for live-cell staining applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies your IVD development with a comprehensive portfolio of high-quality raw materials, including validated antibody pairs and optimized monoclonal antibodies like this CD83 clone. Whether you need bulk reagents for immunology research or rare target sourcing for infectious disease panels, our one-stop supply chain supports every stage from concept to clinic.