Rabbit anti-Mouse CD83 Monoclonal Antibody for Flow Cytometry - O88324

Core Product Specifications and Parameters

Biological Background: CD83 Antigen Function and Localization

• CD83 antigen is a transmembrane glycoprotein belonging to the immunoglobulin superfamily, characterized by an immunoglobulin domain, signal peptide, transmembrane helix, and disulfide bonds.
• Plays a critical role in immune regulation, particularly in CD4(+) T-cell selection, differentiation, and stability, by modulating the E3 ubiquitin ligase MARCHF8, which controls MHC class II trafficking. Related references: PMID:27503071 PMID:27503069
• Also inhibits MARCHF1-mediated ubiquitination of MHC class II, further influencing antigen presentation.
• Acts as an important modulator of protective immune responses against acute infections. Related references: PMID:31527313
• Localized to the cell membrane, consistent with its role in cell surface signaling and MHC class II regulation.
• Abundantly expressed in spleen and brain, and detected in most tissues analyzed, indicating widespread but prominent immune and neural expression.
• Predicted to contain glycosylation sites and disulfide bonds, typical of cell surface glycoproteins.

Experimental Guidance and Technical Tips

• The recombinant immunogen covering amino acids 22-133, which includes the extracellular immunoglobulin-like domain, suggests this antibody may target the surface-exposed region of CD83, suitable for flow cytometry.
• Direct conjugation to allows one-step staining without secondary antibody, reducing background and simplifying protocols.
• For flow cytometry, consider using an appropriate isotype control and blocking Fc receptors to minimize non-specific binding, especially in mouse samples.
• Validation in tissues with high CD83 expression, such as spleen or brain, is recommended to confirm sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers one-stop access to high-quality IVD raw materials, including this rabbit anti-mouse CD83 monoclonal antibody for flow cytometry. Whether you need validated antibody pairs, bulk ancillary reagents, or rare target raw-material sourcing, CamelBio streamlines procurement from concept to clinic. Our comprehensive catalog supports immune function studies with reliable, cost-effective solutions tailored to your development pipeline.