Rabbit anti-Mouse CD83 Monoclonal Antibody for Flow Cytometry - O88324

Core Product Specifications and Parameters

Biological Background: CD83 Function and Localization

• CD83 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily, encoded by the Cd83 gene in mice, and plays diverse roles in immune response regulation.
• It is predominantly expressed on mature dendritic cells and activated lymphocytes, functioning as a key modulator of adaptive immunity by regulating MHC II trafficking.
• CD83 is essential for CD4+ T-cell selection, differentiation, and stability by controlling the activity of the E3 ubiquitin ligase MARCHF8, and it also inhibits MARCHF1-mediated MHC II ubiquitination. Related references: PMID:27503071 PMID:27503069
• The protein acts as an important modulator of protective responses against acute infections, highlighting its role in host defense. Related references: PMID:31527313
• Subcellular localization is at the cell membrane, consistent with its function as a surface receptor involved in intercellular communication.
• Tissue expression is abundant in spleen and brain, with broad detection in most other tissues, indicating widespread physiological importance.
• Predicted molecular weight is 21.3 kDa, and the protein contains an immunoglobulin domain, disulfide bonds, and glycosylation sites, characteristic of immune regulatory molecules.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 22–133) covers the extracellular immunoglobulin-like domain of mouse CD83, likely recognizing the native conformation; consider this when designing blocking or competition experiments.
• This conjugate is optimized for flow cytometry; titrate the antibody and include appropriate isotype and fluorescence minus one (FMO) controls to ensure specific staining.
• CD83 is expressed on dendritic cells and activated lymphocytes; for best detection, use freshly isolated splenocytes or in vitro stimulated immune cells (e.g., LPS-stimulated B cells or CD3/CD28-activated T cells).
• For intracellular staining, a fixation and permeabilization protocol may be necessary, and note that membrane localization could affect access; validate in the relevant sample system.
• Cross-reactivity is specific to mouse; do not assume reactivity with other species unless experimentally confirmed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and researchers with reliable access to IVD raw materials, such as validated monoclonal antibodies like this anti-CD83 reagent, ideal for immune cell profiling assays. Our supply capabilities include optimized antibody pairs, bulk ancillary reagents, and rare target sourcing, supporting your projects from early research to clinical application. Partner with CamelBio to streamline your CD83-target assay development with comprehensive procurement solutions.