Rabbit anti-Mouse CD83 Monoclonal Antibody for FC - O88324

Core Product Specifications and Parameters

Biological Background: CD83 Function and Localization

• CD83 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily, expressed on dendritic cells, lymphocytes, and other immune cells.
• It plays an essential role in CD4+ T-cell selection, differentiation, and stability by regulating the activity of MARCHF8, an E3 ubiquitin ligase that controls MHC II trafficking.
  Related references: PMID:27503071 PMID:27503069
• CD83 inhibits MARCHF1 association with MHC II, thereby reducing MHC II ubiquitination and degradation (By similarity).
• It acts as an important modulator of protective immune responses against acute infections.
  Related references: PMID:31527313
• Subcellular localization: Primarily located on the plasma membrane as a single-pass type I membrane protein.
• Tissue specificity: Abundantly expressed in spleen and brain; also detected in most other tissues.
• Post-translational features: Contains a signal peptide, an Ig-like V-type domain, a transmembrane helix, disulfide bonds, and N-glycosylation sites.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (amino acids 22-133), suggesting the antibody recognizes the native surface antigen, making it well-suited for live-cell flow cytometry.
• For FC applications, titrate the antibody to determine the optimal working concentration for your specific cell type and staining conditions.
• Always include appropriate isotype control antibodies and fluorescence-minus-one (FMO) controls to set gates accurately.
• Since the antibody is directly conjugated to , no secondary antibody is required, simplifying the staining procedure.
• Consider using Fc receptor blocking reagents to reduce nonspecific binding, especially when working with Fc receptor-rich immune cells.
• Validate the staining protocol on known positive and negative mouse cell populations (e.g., mouse splenocytes or dendritic cells) before running experimental samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop supplier of IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research laboratories worldwide. This anti-mouse CD83 monoclonal antibody, with its specificity for a key dendritic cell marker, supports immunological assay development and immune function studies. For rare target sourcing or customized reagent needs, CamelBio provides flexible procurement solutions to bridge your concept-to-clinic journey.