Rabbit anti-Mouse CD82 Monoclonal Antibody for Flow Cytometry - P40237

Core Product Specifications and Parameters

Biological Background: CD82 Function and Localization

• CD82, also known as C33, IA4, Kai1, or Tspan27, is a member of the tetraspanin superfamily and functions as a structural component of tetraspanin-enriched microdomains (TERMs) at the plasma membrane.
• It plays a multifaceted role in regulating cell signal transduction, adhesion, migration, and protein trafficking, particularly by attenuating epidermal growth factor (EGF) signaling through ligand-induced EGFR endocytosis and ubiquitination.
• CD82 enhances cell-matrix adhesion by organizing integrin alpha4/ITA4 at the membrane and suppresses migration mediated by integrins alpha6/ITGA6 and beta1/ITGB1.
• In immune cells, CD82 associates with CD4 or CD8 to deliver costimulatory signals for the TCR/CD3 pathway, restrains phagocyte migration, and supports macrophage activation, including promoting anti-inflammatory phenotypes. Related references: PMID:35754722
• It regulates Toll-like receptor (TLR) signaling by controlling TLR9 trafficking to acidified compartments and subsequent myddosome assembly, and inhibits LPS-induced TLR4 signaling by preventing LPS binding (By similarity). Related references: PMID:31408613
• Independently of TLR signaling, CD82 is recruited to pathogen-containing phagosomes and participates in antigen presentation, and it controls angiogenesis by sequestering VEGFA and PDGFA. Related references: PMID:21149584 PMID:34530889
• Subcellularly, CD82 is located at the cell membrane, cytoplasmic vesicles, and phagosomes. It is post-translationally modified by glycosylation and palmitoylation.
• Tissue expression is highest in the spleen and kidney, with low levels in skeletal muscle and heart.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant protein spanning the N-terminal region (amino acids 1-266) of mouse CD82, which likely includes extracellular domains, consistent with its use in flow cytometry.
• This antibody is validated for flow cytometry (FC) applications; optimize antibody titration and appropriate controls for each experimental system.
• As cross-reactivity is reported for mouse, ensure species specificity when using in multi-species experiments.
• Consider testing the antibody in other applications (e.g., immunofluorescence) if the epitope is accessible in fixed/permeabilized samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, streamlining your procurement of high-quality reagents from discovery to commercialization. Our portfolio includes this anti-CD82 antibody, suited for studying tetraspanin-mediated cellular processes, alongside validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets. Contact us to support your diagnostic assay development and research needs.