Rabbit anti-Mouse CD80 Monoclonal Antibody for Flow Cytometry - Q00609

Core Product Specifications and Parameters

Biological Background: CD80 Function and Localization

• T-lymphocyte activation antigen CD80 (also known as B7-1) is a costimulatory molecule that belongs to the immunoglobulin superfamily and plays an essential role in T-lymphocyte activation. Related references: PMID:16339518, PMID:1714935
• As a primary auxiliary signal, CD80 augments the MHC/TCR signal in naive T-cells by binding to its receptor CD28, leading to activation of downstream pathways including NF-kappa-B and MAPK.
• CD80 also functions as an inhibitor of T-cell activation by serving as a ligand for CTLA4, thereby blocking CD28-mediated T-cell priming.
• CD28/CD80 costimulation stimulates glucose metabolism and ATP synthesis in T-cells via the PI3K/Akt signaling pathway.
• CD80 acts as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses.
• Expressed on B-cells, CD80 is crucial for B-cell/T-cell interactions in both early and late germinal center responses, which are essential for generating effective humoral immune responses. Related references: PMID:22450810
• Subcellular location: Predominantly localized on the cell membrane with single-pass type I membrane protein topology.
• Tissue specificity: Expressed on activated B-cells, gamma interferon-stimulated monocytes, and non-circulating B-cell malignancies.
• Post-translational modifications and structural features: Contains a disulfide bond, N-glycosylation, and an immunoglobulin domain; undergoes alternative splicing. Keywords include 3D-structure, glycoprotein, transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 38-245, encompassing the extracellular domain of mouse CD80, suggesting the antibody can recognize the native conformation on live cells, ideal for flow cytometry.
• For flow cytometry, consider titrating the antibody to determine optimal concentration, and include appropriate isotype controls and Fc receptor blocking steps.
• As the antibody is pre-conjugated with , it can be used directly for staining without the need for secondary detection reagents.
• Ensure proper handling: store the antibody at recommended temperature (shipped on ice bag) and protect from light to maintain fluorophore stability.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we understand the critical demands of diagnostic development and research supply. As your one-stop sourcing bridge, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For immuno-oncology targets like CD80, CamelBio ensures efficient access to rare raw materials, supporting your journey from concept to clinic.