Rabbit anti-Mouse CD80 Monoclonal Antibody for Flow Cytometry - Q00609

Core Product Specifications and Parameters

Biological Background: CD80 Function and Localization

• CD80 (B7-1) is a costimulatory molecule of the immunoglobulin superfamily that is essential for T-lymphocyte activation, serving as the primary auxiliary signal to the MHC/TCR signal in naive T-cells. Related references: PMID:16339518 PMID:1714935
• It functions as a ligand for both CD28, triggering stimulatory pathways such as NF-kappa-B and MAPK, and CTLA4, delivering inhibitory signals that downregulate T-cell responses.
• By binding CD28, CD80 also stimulates T-cell glucose metabolism and ATP synthesis via PI3K/Akt signaling, thereby supporting energetic demands of activated T-cells.
• CD80 modulates the PD-1/PD-L1 pathway by limiting PD-L1 engagement, preventing excessive inhibitory signaling in immune responses.
• Expressed on activated B-cells and interferon-stimulated monocytes, CD80 regulates B-T cell interactions in germinal centers, which is critical for generation of humoral immune responses. Related references: PMID:22450810
• The protein is localized to the cell membrane and undergoes alternative splicing, giving rise to multiple isoforms, along with post-translational modifications including glycosylation.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal working dilution for your cell type and conditions.
• As a directly conjugated antibody (), it eliminates the need for secondary staining, reducing background and simplifying multicolor panel design.
• The immunogen covers amino acids 38-245 of mouse CD80, which corresponds to the extracellular domain; ensure that your target cell population expresses the full-length protein or relevant epitope.
• Validate antibody specificity in your system using appropriate positive and negative controls, such as activated vs. resting B-cells or CD80-transfected cells.
• Consider cell fixation and permeabilization protocols if intracellular staining is desired, as this antibody targets an extracellular epitope by default.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering a wide range of high-quality antibodies, including anti-CD80 clones for immune checkpoint and T-cell activation research. We supply validated products, bulk reagents, and technical support to accelerate your assay development. Whether you need monoclonal antibodies, recombinant proteins, or customized services, CamelBio ensures a seamless pipeline from concept to clinic.