Rabbit anti-Mouse CD80 Monoclonal Antibody for Flow Cytometry - Q00609

Core Product Specifications and Parameters

Biological Background: CD80 Function and Localization

• CD80 is a costimulatory molecule of the immunoglobulin superfamily that plays an essential role in T-lymphocyte activation (PubMed:16339518, 1714935). Related references: PMID:16339518 PMID:1714935
• It serves as the primary auxiliary signal for naive T-cells by binding to CD28, triggering downstream pathways like NF-kappa-B and MAPK.
• CD80 also binds CTLA4, acting as an inhibitor of T-cell activation and providing an immune checkpoint mechanism.
• The CD28/CD80 costimulatory axis stimulates glucose metabolism and ATP synthesis in T-cells via the PI3K/Akt pathway.
• CD80 regulates PDL1/PDCD1 interactions, limiting excessive PD-1-mediated inhibitory signaling in immune responses.
• On B-cells, CD80 is critical for germinal center responses and humoral immunity, regulating B-T cell interactions (PubMed:22450810). Related references: PMID:22450810
• CD80 is localized to the cell membrane and expressed on activated B-cells, interferon-gamma stimulated monocytes, and non-circulating B-cell malignancies.
• The protein features immunoglobulin domains, disulfide bonds, and glycosylation; it exists in alternatively spliced isoforms.

Experimental Guidance and Technical Tips

• This antibody is supplied as a ready-to-use conjugate for flow cytometry. Titrate the antibody to determine the optimal concentration for your specific experimental conditions.
• The immunogen corresponds to the extracellular region (aa 38-245) of mouse CD80, suggesting recognition of the native surface receptor.
• Validate positivity in appropriate mouse cell types, such as activated B-cells or interferon-gamma stimulated monocytes, using relevant controls.
• For multi-color flow cytometry panels, ensure compatibility of with other fluorophores and instrument laser/filter configurations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, supporting diagnostic developers and researchers from concept to clinic. This anti-mouse CD80 antibody is part of our portfolio for T-cell activation and immune checkpoint studies, and we can source validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents tailored to your assay development needs. For rare-target or custom raw-material requirements, our procurement network delivers reliable supply solutions for your projects.