Rabbit anti-Mouse CD79b Monoclonal Antibody for Flow Cytometry - P15530

Core Product Specifications and Parameters

Biological Background: CD79b Function and Localization

• CD79b (also known as B29, Ig-beta, immunoglobulin-associated B29 protein) is the beta chain of the B-cell antigen receptor complex, essential for B-cell signaling.
• Cooperates with CD79A to initiate the signal transduction cascade upon BCR engagement, leading to complex internalization, trafficking to late endosomes, and antigen presentation.
• Enhances phosphorylation of CD79A, likely by recruiting kinases or by protecting CD79A from dephosphorylation.
• Primarily localizes to the cell membrane of B lymphocytes.
• Tissue specificity: expressed specifically in B cells.
• Key structural features include an immunoglobulin domain, a transmembrane helix, and a signal peptide; classified as a glycoprotein and phosphoprotein.
• Post-translational modifications include phosphorylation and ubiquitination (Ubl conjugation).
• Calculated molecular mass is approximately 25.7 kDa.

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to , enabling one-step flow cytometry detection of mouse CD79b on B cells.
• Titrate the antibody in your relevant cell system to determine the optimal staining concentration.
• Consider using an Fc receptor blocking reagent to minimize non-specific binding in flow cytometry.
• The immunogen spans the extracellular domain (aa 26–158), ensuring recognition of surface-expressed CD79b; validate performance with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement experience for diagnostic developers and researchers seeking validated IVD raw materials like this CD79b monoclonal antibody for immune cell profiling. Our supply capabilities encompass optimized antibody pairs, bulk ancillary reagents, and rare target sourcing, supporting your project from concept to clinic. Let CamelBio be your trusted partner in accelerating B-cell research and assay development.