Rabbit anti-Mouse CD72 Monoclonal Antibody for FC - P21855

Core Product Specifications and Parameters

Biological Background: CD72 Function and Localization

• CD72 is a transmembrane glycoprotein and member of the C-type lectin family, primarily expressed on pre-B and mature B lymphocytes but absent from terminally differentiated plasma cells.
• Serves as a co-receptor for the B-cell receptor (BCR), exerting both positive and negative regulatory effects on B-cell activation and differentiation. Related references: PMID:10549631
• Recognizes Sm/ribonucleoprotein (RNP) self-antigen; coligation of CD72 with BCR inhibits BCR signaling. Related references: PMID:27810925
• Inhibitory signaling is mediated by recruitment of PTPN6/SHP-1 phosphatase to the BCR complex upon ligand engagement. Related references: PMID:9590210
• Functions as a ligand for CD5, contributing to the maintenance of regulatory T and B-cell homeostasis. Related references: PMID:24950378 PMID:9723705
• Post-translational modifications include N-linked glycosylation, phosphorylation, and intrachain disulfide bonds.
• UniProt keywords: 3D-structure, Disulfide bond, Glycoprotein, Lectin, Membrane, Phosphoprotein, Receptor, Signal-anchor, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen (extracellular domain aa 226–354) preserves native epitope structure; for flow cytometry, consider using a validated staining protocol on mouse B-cell suspensions.
• Validate antibody specificity with CD72-knockout B cells or competitive blocking with the immunogen peptide to confirm signal.
• Avoid fixation methods that may alter epitope accessibility; titrate the antibody to determine optimal concentration for your experimental system.
• Cross-reactivity is confirmed for mouse; test other species after independent validation if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies the sourcing of IVD raw materials by offering diagnostic-grade monoclonal antibodies, including rare targets like CD72, backed by supply chain reliability and technical consultation. Whether you need validated antibody pairs, bulk ancillary reagents, or customized raw-material support for B-cell immunophenotyping, our one-stop platform streamlines your journey from concept to clinic.