Rabbit anti-Mouse CD72 mAb - P21855

Core Product Specifications and Parameters

Biological Background: CD72 Function and Localization

• CD72 is a B-cell differentiation antigen, also known as Lyb-2, Ly-32, and lymphocyte antigen 32, belonging to the C-type lectin superfamily.
• Acts as a co-receptor of the B cell receptor (BCR), mediating both positive and negative regulatory roles on B-cell functions. Related references: PMID:10549631
• Recognizes the Sm/ribonucleoprotein (RNP) self-antigen ligand; coligation of CD72 with the BCR inhibits BCR signaling. Related references: PMID:27810925
• Ligand binding induces recruitment of PTPN6/SHP-1 to the BCR complex, leading to inhibitory signaling. Related references: PMID:9590210
• Serves as a ligand for CD5, playing a critical role in maintaining regulatory T and B-cell homeostasis. Related references: PMID:24950378, PMID:9723705
• Subcellularly localized to the plasma membrane as a single-pass type II transmembrane protein.
• Expressed specifically in pre-B-cells and mature B-cells, but absent in terminally differentiated plasma cells.
• Post-translational modifications include glycosylation, disulfide bond formation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal extracellular region (amino acids 226–354), which may facilitate recognition of surface-expressed CD72 in flow cytometry applications.
• For FC, consider using standard surface staining protocols on mouse B-cell lines (e.g., A20, WEHI-231) or primary splenocytes; validate antibody titration and isotype controls in the relevant sample system.
• Since CD72 is a co-receptor with modulatory functions, additional functional assays (e.g., co-crosslinking with BCR) may be explored but are not covered by the product’s validated application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop provider of IVD raw materials, offering diagnostic manufacturers and researchers streamlined access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like CD72 in B-cell biology and immunology, we can support your assay development with rare target raw-material sourcing alongside established catalog products. Let CamelBio be your procurement bridge from concept to clinic.