Rabbit anti-Mouse CD72 mAb for FC - P21855

Core Product Specifications and Parameters

Biological Background: CD72 Function and Localization

• CD72, also known as Lyb-2 or Ly-32, is a B-cell differentiation antigen expressed on pre-B-cells and mature B-cells, but absent from terminally differentiated plasma cells.
• It functions as a co-receptor of the B cell receptor (BCR), playing both positive and negative roles in B-cell functions. Related references: PMID:10549631
• CD72 recognizes the Sm/ribonucleoprotein (RNP) self-antigen ligand, and coligation of CD72 with the BCR inhibits BCR signaling. Related references: PMID:27810925
• Ligand binding leads to the recruitment of PTPN6/SHP-1 to the BCR complex, which mediates inhibitory signaling. Related references: PMID:9590210
• CD72 acts as a ligand for CD5, critically maintaining regulatory T and B-cell homeostasis. Related references: PMID:24950378 PMID:9723705
• Subcellularly, CD72 is a single-pass type II membrane protein with its C-type lectin domain exposed extracellularly.
• Structural features include disulfide bonds, glycosylation, and phosphorylation, classifying it as a glycoprotein and phosphoprotein receptor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal extracellular domain (aa 226–354) of mouse CD72, likely enabling recognition of the surface-expressed receptor on live cells by flow cytometry.
• For flow cytometry, consider titrating the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• Use appropriate isotype controls and consider validating specificity with CD72 knockout or knockdown cell lines if available.
• Since CD72 is predominantly expressed on B-lineage cells, co-staining with pan-B-cell markers (e.g., CD19, B220) can help confirm lineage-specific detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw materials, offering comprehensive support for diagnostic manufacturers and research laboratories in immunology and B-cell biology. For CD72-targeted studies and assay development, we provide validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents, along with tailored sourcing for rare targets—helping you streamline your workflow from concept to clinic.