Rabbit anti-Mouse CD71 Monoclonal Antibody for Flow Cytometry - Q62351

Core Product Specifications and Parameters

Biological Background: Transferrin Receptor Protein 1 (CD71) Function and Localization

• Transferrin receptor protein 1 (TfR1, CD71) is encoded by the Tfrc gene in mice and is also known as TR, TFR, p90, TFR1, Trfr, Mtvr1, 2610028K12Rik, and E430033M20Rik.
• Mediates cellular iron uptake via receptor-mediated endocytosis: binds iron-loaded transferrin, internalizes into endosomes where acidification releases iron, then recycles apotransferrin-receptor complex to the surface.
• Essential for erythrocyte and nervous system development (by similarity).
• Positively regulates T and B cell proliferation through iron uptake upon stimulation.
  Related references: PMID:26642240
• Acts as a lipid sensor regulating mitochondrial fusion: low dietary stearate promotes JNK pathway activation, leading to MFN2 degradation; high stearate causes TFRC stearoylation inhibiting JNK, thus preserving MFN2 (by similarity).
• Mediates uptake of NICOL1 into fibroblasts to regulate extracellular matrix production.
  Related references: PMID:38625739
• Localizes to the cell membrane and melanosomes, with additional keywords: disulfide bond, glycoprotein, lipoprotein, palmitate, phosphoprotein, and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , eliminating the need for a secondary antibody in flow cytometry.
• For surface staining, use live, unfixed cells to preserve epitope integrity; avoid permeabilization unless intracellular staining is intended.
• Titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• Include appropriate isotype control and fluorescence minus one (FMO) controls to ensure accurate gating.
• Validate staining in the relevant mouse cell line or primary cells, as reactivity may vary across strains or activation states.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As your sourcing partner, we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies like this anti-CD71 clone, bulk ancillary reagents, and rare target raw-material sourcing to support your iron metabolism, immunology, or oncology research. Let us bridge your procurement needs with high-quality, ready-to-use materials.