Rabbit anti-Mouse CD69 Monoclonal Antibody for FC - P37217

Core Product Specifications and Parameters

Biological Background: CD69 Function and Localization

• CD69 (Early activation antigen CD69), also known as AIM and VEA, is a transmembrane protein belonging to the C-type lectin family and serves as an early activation marker on immune cells.
• It is rapidly upregulated on the surface of platelets, T-lymphocytes, and NK cells upon activation by antigen recognition or cytokine signaling, playing a critical role in immune cell homeostasis (PubMed:20696842, PubMed:38540778). Related references: PMID:20696842, PMID:38540778
• CD69 negatively regulates Th17 cell differentiation through carbohydrate-dependent interaction with galectin-1 on immature dendritic cells and via JAK3/STAT5 signaling that controls RORγt transcription (PubMed:20696842). Related references: PMID:20696842
• The protein also promotes regulatory T-cell conversion by engaging the S100A8/S100A9 complex and acts as an oxidized low-density lipoprotein (oxLDL) receptor in CD4 T-cells, negatively regulating inflammation by inducing PDCD1 expression through NFAT activation.
• In eosinophils, CD69 induces IL-10 production through the ERK1/2 pathway, highlighting its role in modulating inflammatory responses (PubMed:38540778). Related references: PMID:38540778
• CD69 negatively regulates chemotactic responses of effector lymphocytes and dendritic cells to sphingosine 1-phosphate (S1P) by acting as a S1PR1 agonist, promoting receptor internalization and degradation (PubMed:16525420). Related references: PMID:16525420
• Structurally, CD69 is a single-pass type II membrane protein with a C-type lectin domain, featuring disulfide bonds, glycosylation, and phosphorylation sites, consistent with its receptor function.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 64–199 of mouse CD69, corresponding to the extracellular C-type lectin domain, making the antibody suitable for detecting cell surface expression on live, unfixed cells by flow cytometry.
• For flow cytometry, titrate the antibody to determine the optimal working concentration. Include appropriate isotype controls and single-stain compensation controls when using multiple fluorophores.
• Validate antibody performance using known positive controls (e.g., activated mouse T-cells or NK cells) and confirm specificity with CD69-deficient cells or blocking peptides if available.
• The conjugate is compatible with standard red/orange laser lines; ensure instrument settings are appropriately configured to avoid spillover.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Our anti-CD69 antibody supports immunology and immune-oncology research, and we provide integrated technical services to accelerate your diagnostic development from concept to clinic.