Rabbit anti-Mouse CD69 Monoclonal Antibody for FC - P37217

Core Product Specifications and Parameters

Biological Background: CD69 Function and Localization

• CD69, also designated AIM, VEA, or 5830438K24Rik, is a 22.5 kDa type II transmembrane glycoprotein belonging to the C-type lectin family.
• It is rapidly induced on the surface of activated platelets, T-lymphocytes, and NK cells, playing a central role in immune homeostasis (PubMed:20696842, PubMed:38540778). Related references: PMID:20696842 PMID:38540778
• CD69 negatively regulates Th17 cell differentiation through carbohydrate-dependent interaction with galectin-1 and by engaging the JAK3/STAT5 pathway to modulate RORγt expression (PubMed:20696842). Related references: PMID:20696842
• In eosinophils, CD69 triggers IL-10 production via the ERK1/2 pathway (PubMed:38540778). Related references: PMID:38540778
• CD69 serves as a functional antagonist of S1PR1, promoting receptor internalization and thereby dampening lymphocyte and dendritic cell chemotaxis (PubMed:16525420). Related references: PMID:16525420
• The protein localizes to the cell membrane and features disulfide bonds, glycosylation, and phosphorylation sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 64–199), making this antibody suitable for detecting surface CD69 in flow cytometry.
• As CD69 is rapidly upregulated upon activation, consider using appropriate stimulation conditions (e.g., PMA/ionomycin) and including unstimulated controls to verify specificity.
• Optimize antibody titration in the relevant mouse immune cell populations (splenocytes, lymph node cells) and validate with isotype controls for accurate gating.
• For intracellular detection, permeabilization may be necessary; confirm compatibility with fixation/permeabilization protocols beforehand.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like this anti-CD69 clone for flow cytometry applications. As a one-stop sourcing partner, we support projects from target selection to clinical validation, offering bulk antibody pairs, ancillary reagents, and custom development services. Whether you need standard reagents or rare target raw materials, our supply chain ensures reliable access to accelerate your diagnostic development.