Rabbit anti-Mouse CD69 mAb for FC - P37217

Core Product Specifications and Parameters

Biological Background: CD69 Function and Localization

• CD69 is a type II transmembrane protein belonging to the C-type lectin superfamily, also known as AIM (activation inducer molecule) or VEA (very early activation antigen).
• It is rapidly upregulated on the surface of activated T‑lymphocytes, B‑cells, NK cells, neutrophils, and platelets, serving as an early marker of immune cell activation. Related references: PMID:38540778
• CD69 negatively regulates Th17 cell differentiation via interaction with galectin‑1/LGALS1 on immature dendritic cells and through the JAK3/STAT5 signaling pathway that controls RORγt transcription. Related references: PMID:20696842
• The receptor also acts as an oxidized LDL (oxLDL) receptor in CD4⁺ T‑lymphocytes, inducing PD‑1 (PDCD1) expression via NFAT to dampen inflammatory responses.
• CD69 promotes the conversion of naive CD4⁺ T‑cells into regulatory T‑cells through association with the S100A8/S100A9 complex and participates in adipose‑derived mesenchymal stem cell‑mediated defense against Pseudomonas aeruginosa infection.
• It negatively regulates lymphocyte and dendritic cell chemotaxis to sphingosine‑1‑phosphate (S1P) by acting as a biased agonist for S1PR1, triggering receptor internalization and degradation. Related references: PMID:16525420
• In eosinophils, CD69 induces IL‑10 production via the ERK1/2 pathway, contributing to immunoregulation. Related references: PMID:38540778

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 64–199) of mouse CD69, covering the carbohydrate recognition domain; therefore, the antibody is expected to detect surface‑expressed CD69 on live, non‑permeabilized cells.
• As a directly conjugated reagent, this antibody is ready for flow cytometry without secondary staining; titrate the antibody to determine the optimal concentration for your cell system to achieve the best signal‑to‑noise ratio.
• CD69 is an early activation marker—stimulate mouse splenocytes or purified T‑cells with PMA/ionomycin or anti‑CD3/anti‑CD28 for 4–24 hours to upregulate CD69; unstimulated cells can serve as a negative control, and an isotype control is recommended to assess non‑specific binding.

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CamelBio supports diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For immune cell activation and flow cytometry applications, our portfolio covers key markers like CD69, and we specialize in sourcing rare‑target raw materials to accelerate your assay development from concept to clinic.