Rabbit anti-Mouse CD68 Monoclonal Antibody for Intracellular Flow Cytometry - P31996

Core Product Specifications and Parameters

Biological Background: CD68 Function and Localization

• CD68 (macrosialin, also known as Lamp4, gp110, Scard1) is a heavily glycosylated transmembrane protein belonging to the lysosomal-associated membrane protein (LAMP) family.
• It is predominantly expressed in tissue macrophages and, to a lesser extent, in dendritic cells.
• CD68 plays a role in phagocytic activities of macrophages, participating in both intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions.
• It facilitates macrophage homing to specific tissue sites by binding to tissue- and organ-specific lectins or selectins.
• The protein rapidly recycles between endosomes, lysosomes, and the plasma membrane, a process that may support macrophage crawling over selectin-bearing substrates.
• At the subcellular level, CD68 localizes primarily to endosome and lysosome membranes, with some plasma membrane expression.
• Structural features include a signal peptide, a transmembrane helix, multiple disulfide bonds, and N-linked glycosylation sites, consistent with its lysosomal targeting and stability.

Experimental Guidance and Technical Tips

• The immunogen encompasses the luminal domain of CD68 (aa 21-291); this region is expected to be exposed after permeabilization, making the antibody suitable for intracellular flow cytometry.
• For FC (intra), optimize fixation and permeabilization steps for your specific macrophage or dendritic cell type; consider employing an appropriate isotype control to assess background.
• Due to heavy glycosylation of CD68, denaturing conditions or certain fixation protocols may alter epitope recognition; preliminary titration experiments are recommended.
• While validated for flow cytometry, the antibody may also be compatible with immunohistochemistry or ELISA if the epitope remains accessible; prospective users should validate in their target sample systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic developers from early R&D to clinical application. For macrophage biology and CD68-related studies, we supply validated antibody pairs, bulk reagents, and technical support. Our catalog includes optimized monoclonal/polyclonal antibodies, custom production, and rare target procurement to accelerate your workflow from concept to clinic.