Rabbit anti-Mouse CD68 Monoclonal Antibody for IF/ICC, FC - P31996

Core Product Specifications and Parameters

Biological Background: Macrosialin (CD68) Function and Localization

• Macrosialin (CD68) is a heavily glycosylated type I transmembrane protein belonging to the LAMP family, predominantly expressed in tissue macrophages and to a lesser extent in dendritic cells.
• It plays a key role in phagocytic activities of macrophages, participating in both intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions.
• CD68 can bind to tissue- and organ-specific lectins or selectins, facilitating the homing of macrophage subsets to particular sites.
• Rapid recycling of CD68 from endosomes and lysosomes to the cell membrane may enable macrophages to crawl over selectin-bearing substrates or other cells.
• The protein is localized to endosome membranes, lysosome membranes, and the cell membrane, consistent with its endocytic trafficking function.
• Multiple isoforms are produced by alternative splicing, and the protein features disulfide bonds and extensive glycosylation, contributing to its structural stability and immune recognition.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 21-291) of mouse CD68, suggesting the antibody likely recognizes an exposed epitope suitable for surface staining in flow cytometry and immunofluorescence applications.
• For flow cytometry, surface staining of live cells may be feasible without permeabilization, but it is advisable to validate staining patterns in both unpermeabilized and permeabilized macrophage samples.
• In immunofluorescence/immunocytochemistry, mild fixation and permeabilization conditions (e.g., 4% PFA followed by 0.1% Triton X-100) should allow detection of the total cellular CD68 pool, including endosomal/lysosomal compartments.
• Consider using this antibody to identify macrophage populations in murine tissues, such as spleen and lymph node, with appropriate isotype controls.
• Due to the dye conjugation, avoid freeze-thaw cycles and protect from prolonged light exposure to maintain fluorescence intensity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials like this anti-CD68 antibody, ideal for macrophage and immunological studies. Beyond single reagents, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your research and development pipeline. Partner with us to bridge the gap from concept to clinical application.