Rabbit anti-Mouse CD68 mAb for IF/ICC and FC - P31996

Core Product Specifications and Parameters

Biological Background: CD68 Function and Localization

• CD68, also known as macrosialin, Lamp4, gp110, or Scard1, is a heavily glycosylated transmembrane protein belonging to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family.
• It is predominantly expressed in tissue macrophages and, to a lesser extent, in dendritic cells, serving as a classic marker for these phagocytic cell types.
• CD68 plays a role in phagocytic activities, participating in intracellular lysosomal metabolism and mediating extracellular cell-cell and cell-pathogen interactions.
• The protein binds tissue- and organ-specific lectins or selectins, facilitating the homing of macrophage subsets to specific sites within the body.
• CD68 rapidly recycles between endosomes, lysosomes, and the plasma membrane, a process that may enable macrophages to crawl over selectin-bearing substrates or other cells.
• Subcellularly, CD68 localizes to endosome membranes, lysosome membranes, and the cell membrane, consistent with its dynamic trafficking and functional roles.
• The protein contains a signal peptide, a large luminal/extracellular domain with mucin-like repeats, a single transmembrane helix, and a short cytoplasmic tail, and it is subject to alternative splicing and disulfide bond formation.

Experimental Guidance and Technical Tips

• For immunofluorescence (IF/ICC), consider including a permeabilization step (e.g., with Triton X-100 or saponin) to detect intracellular CD68 pools in lysosomes/endosomes, as the protein resides in these compartments.
• For flow cytometry (FC), test both live and fixed/permeabilized cell protocols to capture surface-localized and total CD68 expression. Titrate the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions.
• Use appropriate isotype controls and fluorescence minus one (FMO) controls to confirm staining specificity, especially when analyzing macrophage and dendritic cell populations.
• Since the immunogen spans amino acids 21–291 of mouse CD68 (covering the extracellular/luminal domain), the antibody is expected to recognize the native protein under both denaturing and native conditions, but confirm performance in your specific application via pilot experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a strategic one-stop sourcing partner providing diagnostic manufacturers and research labs with high-quality IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This anti-mouse CD68 antibody supports advanced macrophage and dendritic cell research, and we offer rapid access to optimized monoclonal antibodies, rare target sourcing, and technical consulting to streamline your assay development.