Rabbit anti-Mouse CD62L Monoclonal Antibody for Flow Cytometry - P18337

Core Product Specifications and Parameters

Biological Background: L-selectin Function and Localization

• L-selectin (CD62L, Ly-22) is a calcium-dependent (C-type) lectin that mediates cell adhesion by binding to glycoprotein ligands on neighboring cells, playing a central role in leukocyte trafficking. Related references: PMID:1693096
• It is a transmembrane glycoprotein, with an N-terminal C-type lectin domain, an EGF-like domain, two sushi (CCP/SCR) domains, a transmembrane region, and a short cytoplasmic tail (based on UniProt keywords and domain annotations).
• L-selectin is predominantly expressed on the surface of leukocytes, including lymphocytes, and is required for lymphocyte homing by mediating adhesion to high endothelial venules (HEV) in peripheral lymph nodes.
• Upon leukocyte activation, L-selectin undergoes rapid proteolytic shedding from the cell surface, which regulates leukocyte rolling and transmigration.
• It also participates in the initial tethering and rolling of leukocytes along the activated endothelium at sites of inflammation, a prerequisite for firm adhesion and extravasation.
• Subcellularly, L-selectin is localized to the cell membrane and is clustered on the tips of microvilli, which facilitates its interaction with ligands under flow conditions.
• Gene family: SELL belongs to the selectin family (SELL, SELE, SELP), which are type I transmembrane glycoproteins that share a similar domain architecture and mediate cell–cell interactions in the vascular system.
• Tissue specificity: Primarily expressed in lymphoid tissues, consistent with its role in lymphocyte recirculation and immune surveillance.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular region (aa 39–332) of mouse CD62L, encompassing the lectin and EGF-like domains that are likely exposed on the cell surface; thus, the resulting monoclonal antibody is well-suited for detecting native CD62L on live cells by flow cytometry.
• As a recombinant rabbit monoclonal antibody, it offers high lot-to-lot consistency and affinity, minimizing variability in sensitive applications.
• The conjugate provides a far-red fluorescent label with high brightness and photostability, ideal for multicolor flow cytometry panels with minimal overlap in the visible range.
• Since CD62L is rapidly shed after cell stimulation, consider optimizing sample preparation steps (e.g., temperature, stimulation time) to preserve surface expression and ensure reproducible staining.
• Although the predicted molecular weight is 42 kDa, the mature glycosylated protein may migrate at a higher apparent molecular weight in SDS-PAGE; if adapting for Western blot, validate under appropriate reducing conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research institutions with reliable antibody products like this anti-mouse CD62L monoclonal antibody. Whether you require validated antibody pairs, purified bulk reagents, or assistance in sourcing rare targets for leukocyte adhesion and inflammation research, we streamline procurement from concept to clinic. Our global supply network ensures consistent quality and support for your assay development needs.