Rabbit anti-Mouse CD62L Monoclonal Antibody for Flow Cytometry - P18337

Core Product Specifications and Parameters

Biological Background: L-selectin Function and Localization

• L-selectin (CD62L, LAM-1, LECAM1, Ly-22, MEL-14 antigen) is a calcium-dependent lectin that mediates cell adhesion by binding glycoproteins on adjacent cells, crucial for lymphocyte homing to high endothelial venules in lymph nodes. Related references: PMID:1693096
• This transmembrane protein promotes initial tethering and rolling of leukocytes on endothelial cells, a key step in immune cell trafficking and inflammation (By similarity).
• It is a member of the selectin family with characteristic EGF-like, sushi repeat, and lectin domains, as well as a transmembrane helix.
• Predominantly expressed in lymphoid tissues, it functions as a lymph node homing receptor guiding lymphocyte migration.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, which are essential for its stability and function.
• The mouse L-selectin protein has a molecular weight of approximately 42 kDa (42288 Da).

Experimental Guidance and Technical Tips

• For flow cytometry, prepare single-cell suspensions from mouse lymphoid tissues (such as lymph nodes or spleen) and consider titrating the antibody (e.g., 0.1–1 µg per 10^6 cells) to optimize staining.
• The recombinant protein immunogen may generate antibodies that recognize native epitopes; validate cross-reactivity with other relevant species if needed.
• Use appropriate isotype control antibodies and viability dyes to distinguish specific binding.
• For optimal results, follow standard flow cytometry protocols, including Fc receptor blocking if necessary to reduce non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive selection of IVD raw materials, including validated monoclonal antibodies like this anti-CD62L antibody for flow cytometry-based lymphocyte analysis. Our portfolio encompasses optimized antibody pairs, bulk ancillary reagents, and rare target sourcing to streamline your assay development pipeline. Rely on CamelBio for reliable, high-quality raw materials from concept to clinic.