Rabbit anti-Mouse CD6 Monoclonal Antibody for FC - Q61003

Core Product Specifications and Parameters

Biological Background: CD6 Function and Localization

• CD6 is a T-cell differentiation antigen and cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses. It interacts with ALCAM/CD166 and contributes to signaling cascades triggered by the TCR/CD3 complex. Related references: PMID:24584089
• It functions as a costimulatory molecule, promoting T-cell activation and proliferation, and contributes to the formation and maturation of the immunological synapse.
• CD6 acts as a calcium-dependent pattern receptor that binds both Gram-positive and Gram-negative bacteria, recognizing LPS and lipoteichoic acid. This binding activates MAP kinase signaling and induces pro-inflammatory cytokine secretion.
• Subcellular localization: Predominantly localized to the cell membrane, consistent with its role in cell adhesion and signaling.
• Tissue specificity: Expressed predominantly in thymus, lymph node, and spleen, reflecting its role in T-cell biology and immune tissues.
• Post-translational modifications and structural features: CD6 is a glycoprotein with disulfide bonds and phosphorylation sites, featuring alternative splicing, a signal peptide, and a transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 20–155), likely including the ligand-binding region, making it suitable for detecting cell surface CD6 by flow cytometry.
• For flow cytometry, consider titrating the antibody to determine the optimal staining concentration for your specific sample type and experimental conditions.
• Validate the antibody in relevant mouse tissue or cell lines (e.g., thymocytes, splenocytes, or lymph node cells) to confirm specificity and signal-to-noise ratio.
• Since CD6 may be expressed at varying levels depending on T-cell activation status, include appropriate positive and negative controls to ensure assay sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering diagnostic manufacturers and research institutions streamlined access to critical reagents like this CD6 antibody. Our extensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target sourcing to accelerate your T-cell research and immunoassay development. Partner with CamelBio to efficiently bridge your raw-material needs from concept to clinical application.