Rabbit anti-Mouse CD59a Monoclonal Antibody for FC - O55186

Core Product Specifications and Parameters

Biological Background: CD59a Function and Localization

• CD59a glycoprotein, also known as protectin, MAC-inhibitory protein (MAC-IP), or MACIF, is a GPI-anchored inhibitor of the complement membrane attack complex (MAC).
• It acts by binding to C8 components of the assembling MAC, preventing C9 polymerization and thereby protecting self-cells from complement-mediated lysis.
• The protein is expressed on the cell membrane and also found in a secreted form.
• CD59a is broadly expressed across tissues including liver, kidney, spleen, thymus, brain, and heart, as well as on mononuclear cells, erythrocytes, and platelets; it is barely detected in neutrophils.
• Key features include a disulfide bond-rich structure, N-glycosylation, and a GPI-anchor that localizes it to lipid rafts.
• As a reference proteome member, CD59a plays a critical role in immune homeostasis and self-tolerance.

Experimental Guidance and Technical Tips

• This antibody is validated for flow cytometry; consider titrating to determine the optimal working concentration for your specific cell type and experimental conditions.
• The immunogen lies within the extracellular domain (aa 24-96), which is exposed and involved in MAC binding; it is expected to recognize native CD59a on live, unfixed cells.
• Include appropriate isotype controls and Fc receptor blocking steps to minimize nonspecific binding, especially when working with Fc-receptor-rich cells.
• GPI-anchored proteins can be cleaved by PI-PLC; if surface staining appears low, assess the integrity of the GPI anchor in your experimental setting.
• For best results, use fresh or properly cryopreserved cells to maintain membrane integrity and avoid antigen shedding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering validated monoclonal antibodies like this anti-mouse CD59a clone to support complement pathway research and diagnostic applications. From optimized antibody pairs and bulk conjugates to ancillary reagents, we streamline procurement for diagnostic manufacturers—backed by technical expertise to accelerate your project from concept to clinic.